TEM: sucrose in cryo-specimens

[Sacha De Carlo]

Dear people,

there is nice data in the literature about sucrose/trehalose comparison 
for thin film preparations for cryo-em, see the following :

De Carlo S., Adrian M., Kälin P., Mayer J. M. and Dubochet J. (1999) 
Unexpected property of trehalose as observed by cryo-electron 
microscopy. Journal of Microscopy, 196, 40-45.

Yours sincerely,

Sacha


Le Mercredi, 29 jan 2003, à 18:08 Europe/Zurich, D. Prabha Dias, PhD a 
écrit :

> I've been able to freeze samples with >5% sucrose by washing the 
> sample once on the grid.  I would think 1% sucrose would wash out very 
> easily, if you need to do it at all. As for image quality, I can only 
> tell you that they are qualitatively comparable to samples without 
> sucrose.  Does anyone have any data on this?
>
> -Prabha
>
> At 15:49 +0100 1/29/03, Philip Koeck wrote:
>> Me again,
>>
>> would 1% sucrose be a problem in frozen hydrated specimens (single
>> particles)?
>> If so is it necessary to dyalize it out or is it sufficient to wash 
>> it of
>> the grid before
>> plunge-freezing?
>>
>> Thanks in advance,
>>
>> Philip
>>
>> Philip Koeck
>> Svdertvrns Hvgskola and
>> Karolinska Institutet
>> Dept. of Bioscience at Novum
>> S-14157 Huddinge
>> Sweden
>> phone: +46-8-6089186
>> fax: +46-8-6089290
>
> -- 
> D. Prabha Dias, Ph.D.
> Division of Biology, California Institute of Technology
> M/C 114-96, BIO19
> 1200 East California Blvd.
> Pasadena, CA 91125
>
> phone: (626)395-8829
> fax: (626)395-5730
> email: prabha at caltech.edu
>
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==================================================
Sacha De Carlo, Ph.D
Département de Génomique et Biologie Structurales
Institut de Génétique et Biologie Moléculaire et Cellulaire
UMR 7104 - ULP Strasbourg
1, rue Laurent Fries
F--67404 ILLKIRCH
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