TEM: sucrose in cryo-specimens

[Philip Koeck]

Me again,

would 1% sucrose be a problem in frozen hydrated specimens (single
particles)?
If so is it necessary to dyalize it out or is it sufficient to wash it of
the grid before
plunge-freezing?

Thanks in advance,

Philip

Philip Koeck
Svdertvrns Hvgskola and
Karolinska Institutet
Dept. of Bioscience at Novum
S-14157 Huddinge
Sweden
phone: +46-8-6089186
fax: +46-8-6089290

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