Negative stain

[Carmen A. Mannella]

If the objective is to image the macromolecular density in a specimen,
negative stain (cryo or otherwise) is not the way to go.  With it, you
image the stain not the protein, nucleic acid, lipid, etc.  If you want
outlines of the specimen, it is very useful, with the proviso that
different metal salts give varying degrees of positive staining of select
features, which can complicate interpretation of the object, especially at
high resolution (below 2 nm or so).

Carmen Mannella


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