Largest cryo-neg stain differences?

[reinhard rachel]

> Why you guys do not consider to use cryo-negative staining ?
>
> We could show that cryo-negative staining preserves the structure with
> full or at least partial hydration in some cases.
>
> If one is interested, see "De Carlo et al. (2002). Journal of Struct.
> Biol 138, 216-226"

I agree .... and also had excellent experiences with cryo-negative
staining:
R.Rachel et al. (1986) Ultramicrosopy 20:305-316
(Resolution: 0.8 nm)

Yours sincerely,
Reinhard Rachel

Univ.Regensburg.
Lehrstuhl fuer Mikrobiologie
D-93053 Regensburg
Germany
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