[3dem] 3dem Digest, Vol 217, Issue 8

Tue Sep 9 10:24:20 PDT 2025

Hello Len,
I’ve personally not seen ethane used for plunge freezing expire. I’ve seen tanks older than at least five years working fine, but I’ve not done a survey before on this topic. As long as the tank holds pressure, I’d expect it to expel any potential external contamination. I suppose some internal lining or gaskest or something could start degrading into the pressurized volume, but I’ve not heard of such a thing for any kind of gas tank.

Sincerely,
Seth

Seth Villarreal, PhD
Advanced Workflow Specialist – Sample Preparation

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Today's Topics:

   1. Postdoctoral Scientist Position in Biological Timing

      Mechanisms (Halil Aydin)

   2. Webinar: Meet the new Talos 12 TEM - 18th Sept (Naylor, Claire E.)

   3. Ethane Cylinder Age (Thomas, Leonard M.)

----------------------------------------------------------------------

Message: 1

Date: Mon, 8 Sep 2025 14:54:30 -0400

From: Halil Aydin <ha3416 at nyu.edu<mailto:ha3416 at nyu.edu>>

To: 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>

Subject: [3dem] Postdoctoral Scientist Position in Biological Timing

               Mechanisms

Message-ID:

               <CAJ_3dUEkzHZh7m4LiugXW7urMq+UA2nEvO6ugMm=tTcAx-DxEg at mail.gmail.com<mailto:CAJ_3dUEkzHZh7m4LiugXW7urMq+UA2nEvO6ugMm=tTcAx-DxEg at mail.gmail.com>>

Content-Type: text/plain; charset="utf-8"

Dear EM community,

A joint postdoctoral position is available in my laboratory at New York

University (https://urldefense.com/v3/__https://www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$<https://urldefense.com/v3/__https:/www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$>) and Dr. Mustafa Aydogan's laboratory at

Columbia University (https://urldefense.com/v3/__https://aydoganlab.com/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_GNY7edAw$<https://urldefense.com/v3/__https:/aydoganlab.com/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_GNY7edAw$>).

We are investigating the structural and cellular mechanisms that govern

time control in development and developmental metabolism, and seeking

candidates who are familiar with electron cryo-microscopy (cryo-EM)

technologies, biochemical tools, and structural cell biology approaches.

Please see

https://urldefense.com/v3/__https://hhmi.wd1.myworkdayjobs.com/en-US/External/job/Postdoctoral-Scientist---Aydogan-Lab_R-3797__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F5BXP0IQ$<https://urldefense.com/v3/__https:/hhmi.wd1.myworkdayjobs.com/en-US/External/job/Postdoctoral-Scientist---Aydogan-Lab_R-3797__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F5BXP0IQ$>

for

details and to apply.

Best,

Halil

--

Halil Aydin, Ph.D.

Assistant Professor

Metabolism and Inflammation Research

NYU Pain Research Center

Department of Molecular Pathobiology

New York University College of Dentistry

Schwartz Hall, 9th Floor, Room 902C

345 East 24th Street, New York, NY 10010

Website: https://urldefense.com/v3/__https://www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$<https://urldefense.com/v3/__https:/www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$>

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------------------------------

Message: 2

Date: Tue, 9 Sep 2025 10:28:44 +0000

From: "Naylor, Claire E." <claire.naylor at thermofisher.com<mailto:claire.naylor at thermofisher.com>>

To: "'3dem at ncmir.ucsd.edu'" <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>

Subject: [3dem] Webinar: Meet the new Talos 12 TEM - 18th Sept

Message-ID:

 <SJ0P222MB0186DA39D31060E29F9C477A860FA at SJ0P222MB0186.NAMP222.PROD.OUTLOOK.COM<mailto:SJ0P222MB0186DA39D31060E29F9C477A860FA at SJ0P222MB0186.NAMP222.PROD.OUTLOOK.COM>>

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Hi,

   Pleased to share details of our latest webinar:

Meet the new Talos 12 TEM

18 September 2025

Register here: https://urldefense.com/v3/__https://www.thermofisher.com/uk/en/home/events/meet-the-new-talos-12-tem.html__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhkyK7dGo$<https://urldefense.com/v3/__https:/www.thermofisher.com/uk/en/home/events/meet-the-new-talos-12-tem.html__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhkyK7dGo$>

Session 1: September 18, 10 a.m. CST/BJT, 11 a.m. JST, 12 p.m. AEST

Session 2: September 18, 2 p.m. CEST, 8 a.m. EDT

Session 3: September 18, 2 p.m. EDT, 11 a.m. PDT

The user-friendly Thermo Scientific Talos 12 TEM offers exceptional performance for a diverse range of scientific imaging and experimentation needs. It features a reduced footprint, a robust enclosure, and systems that improve accessibility, bringing trusted performance and automation to more researchers and their labs.

In this webinar, you will learn about:

  *   Recent advancements in 120 kV TEM hardware and software that improve accessibility and automation

  *   How built-in automation enables high-throughput, unattended batch acquisition of multi-scale data

  *   New workflows enhanced with AI-training algorithms designed to accelerate and automate high-throughput acquisition and analysis of nanoparticle data under cryogenic conditions

Join us to explore how these advancements make traditional TEM technology more accessible, efficient, and powerful for a wider range of users in life sciences and clinical research.

About the speakers

Tim Booth, Product Marketing Manager, Thermo Fisher Scientific

With 23 years of experience in cryo-EM, Tim?s expertise encompasses structural biology, workflow strategy, and core facility management. In his current role, he is responsible for promoting and supporting accessible solutions that lower barriers for those new to TEM and cryo-EM and provide the most straightforward and economical approach to reaching higher data quality and structural resolutions.

Ond?ej Vyroubal, Engineer, Thermo Fisher Scientific

Ond?ej holds a master's degree in physics engineering and nanotechnology from Brno University of Technology. He has a strong background in R&D with eight years at Thermo Fisher Scientific, where he helped develop advanced cameras and detectors and now leads the technical architecture of the Talos 12 TEM.?

Reint Boer Iwema, Software Product Manager, Thermo Fisher Scientific

After obtaining?his master's degree in chemistry, Reint developed cryo-EM and X-ray crystallography software at Imperial College London and Leiden University. He previously worked on medical imaging software and currently leads software development for the cryo-electron tomography workflow on FIB-SEM and TEM?systems.

Look forward to seeing some of you there,

Claire

Claire Naylor, Ph.D (she/her/hers)

Regional Market Development EMEA

Thermo Fisher Scientific

Stafford House, 1 Boundary Park, Hemel Hempstead Industrial Estate | HP2 7GE | UK

Claire.naylor at thermofisher.com<mailto:Claire.naylor at thermofisher.com<mailto:Claire.naylor at thermofisher.com%3cmailto:Claire.naylor at thermofisher.com>> | https://urldefense.com/v3/__http://www.thermofisher.com__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhSYImygg$<https://urldefense.com/v3/__http:/www.thermofisher.com__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhSYImygg$><https://urldefense.com/v3/__http://www.thermofisher.com/__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhh5rHkrc$<https://urldefense.com/v3/__http:/www.thermofisher.com/__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhh5rHkrc$>>  | Mobile: +44 (0)7353 153679

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------------------------------

Message: 3

Date: Tue, 9 Sep 2025 15:55:51 +0000

From: "Thomas, Leonard M." <lmthomas at ou.edu<mailto:lmthomas at ou.edu>>

To: "'3dem at ncmir.ucsd.edu'" <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>

Subject: [3dem] Ethane Cylinder Age

Message-ID:

 <SA1PR03MB634074F9B5B4B609D318D9E0B70FA at SA1PR03MB6340.namprd03.prod.outlook.com<mailto:SA1PR03MB634074F9B5B4B609D318D9E0B70FA at SA1PR03MB6340.namprd03.prod.outlook.com>>

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Hello All,

Sort of oddish question but how long is a research grade Ethane cylinder good for?  We have one that is probably at least 6 years old and I was wondering when I should worry about the quality of the ethane for plunge freezing.  It really was not used much when it was first purchased but has been since we got a hold of it.

Thank in advance for any thoughts,

Len

Leonard M. Thomas, Ph.D.

Biomolecular Structure Core Director

Oklahoma COBRE in Structure Biology

Department of Chemistry and Biochemistry

Stephenson Life Science Research Center

101 Stephenson Parkway

Norman, OK 73019

(405)325-1126

https://urldefense.com/v3/__https://www.ou.edu/structuralbiology/cobre-core-facilities/mcl__;!!Mih3wA!DO0wJMyTsaMFQOwuJ7rLyohXQv3uYiN8RO0vsX_tMhhE9EWUMoK9DC5af0LS758PNfUK3-1O0-zOMb3vY78$<https://urldefense.com/v3/__https:/www.ou.edu/structuralbiology/cobre-core-facilities/mcl__;!!Mih3wA!DO0wJMyTsaMFQOwuJ7rLyohXQv3uYiN8RO0vsX_tMhhE9EWUMoK9DC5af0LS758PNfUK3-1O0-zOMb3vY78$>

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