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<p class="MsoNormal"><span style="font-size:12.0pt">Hello Len,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">I’ve personally not seen ethane used for plunge freezing expire. I’ve seen tanks older than at least five years working fine, but I’ve not done a survey before on this topic. As long as the tank holds pressure,
I’d expect it to expel any potential external contamination. I suppose some internal lining or gaskest or something could start degrading into the pressurized volume, but I’ve not heard of such a thing for any kind of gas tank.
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Sincerely,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Seth<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:black;mso-ligatures:standardcontextual">Seth Villarreal, PhD<br>
</span></b><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black;mso-ligatures:standardcontextual">Advanced Workflow Specialist – Sample Preparation
<br>
<br>
<a href="mailto:seth.villarreal@leica-microsystems.com"><span style="color:#0563C1">seth.villarreal@leica-microsystems.com</span></a></span><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#666666;mso-ligatures:standardcontextual"><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#666666;mso-ligatures:standardcontextual">Leica Microsystems Inc.<br>
30 Parkway N. Blvd., Suite 300, Deerfield, IL 60015 United States<o:p></o:p></span></p>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Today's Topics:<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> 1. Postdoctoral Scientist Position in Biological Timing<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> Mechanisms (Halil Aydin)<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> 2. Webinar: Meet the new Talos 12 TEM - 18th Sept (Naylor, Claire E.)<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> 3. Ethane Cylinder Age (Thomas, Leonard M.)<o:p></o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">----------------------------------------------------------------------<o:p></o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Message: 1<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Date: Mon, 8 Sep 2025 14:54:30 -0400<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">From: Halil Aydin <<a href="mailto:ha3416@nyu.edu">ha3416@nyu.edu</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">To: <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Subject: [3dem] Postdoctoral Scientist Position in Biological Timing<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> Mechanisms<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Message-ID:<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> <<a href="mailto:CAJ_3dUEkzHZh7m4LiugXW7urMq+UA2nEvO6ugMm=tTcAx-DxEg@mail.gmail.com">CAJ_3dUEkzHZh7m4LiugXW7urMq+UA2nEvO6ugMm=tTcAx-DxEg@mail.gmail.com</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Content-Type: text/plain; charset="utf-8"<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Dear EM community,<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">A joint postdoctoral position is available in my laboratory at New York<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">University (<a href="https://urldefense.com/v3/__https:/www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$">https://urldefense.com/v3/__https://www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$</a>) and Dr. Mustafa Aydogan's laboratory at<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Columbia University (<a href="https://urldefense.com/v3/__https:/aydoganlab.com/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_GNY7edAw$">https://urldefense.com/v3/__https://aydoganlab.com/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_GNY7edAw$</a>).<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">We are investigating the structural and cellular mechanisms that govern<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">time control in development and developmental metabolism, and seeking<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">candidates who are familiar with electron cryo-microscopy (cryo-EM)<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">technologies, biochemical tools, and structural cell biology approaches.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Please see<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><a href="https://urldefense.com/v3/__https:/hhmi.wd1.myworkdayjobs.com/en-US/External/job/Postdoctoral-Scientist---Aydogan-Lab_R-3797__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F5BXP0IQ$">https://urldefense.com/v3/__https://hhmi.wd1.myworkdayjobs.com/en-US/External/job/Postdoctoral-Scientist---Aydogan-Lab_R-3797__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F5BXP0IQ$</a><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">for<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">details and to apply.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Best,<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Halil<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">-- <o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Halil Aydin, Ph.D.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Assistant Professor<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Metabolism and Inflammation Research<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">NYU Pain Research Center<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Department of Molecular Pathobiology<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">New York University College of Dentistry<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Schwartz Hall, 9th Floor, Room 902C<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">345 East 24th Street, New York, NY 10010<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Website: <a href="https://urldefense.com/v3/__https:/www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$">https://urldefense.com/v3/__https://www.aydinlab.org/__;!!Mih3wA!GxcN6Bb__2NHl5gtcpvk_SEESE2qfBWlcZa5Onzoy_38tsl1RJh15CXCoFLwZdPbkXbtnPNYZ_F6qn1Zcg$</a><o:p></o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">URL: <<a href="https://urldefense.com/v3/__http:/mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20250908/e6a7d9b1/attachment-0001.html__;!!G8Er-fQLpA!JnBXJdzYclzZzGvIsPdOhMvs-dmJ8njYpfbOwlBeyhgaIlTWei80ydJlQ7d4NC-k-LJX8jJLge-pRGdFjxaPXKN-Iu7jVDhRiOqMEwXmoww$">https://urldefense.com/v3/__http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20250908/e6a7d9b1/attachment-0001.html__;!!G8Er-fQLpA!JnBXJdzYclzZzGvIsPdOhMvs-dmJ8njYpfbOwlBeyhgaIlTWei80ydJlQ7d4NC-k-LJX8jJLge-pRGdFjxaPXKN-Iu7jVDhRiOqMEwXmoww$</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">------------------------------<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Message: 2<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Date: Tue, 9 Sep 2025 10:28:44 +0000<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">From: "Naylor, Claire E." <<a href="mailto:claire.naylor@thermofisher.com">claire.naylor@thermofisher.com</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">To: "'3dem@ncmir.ucsd.edu'" <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Subject: [3dem] Webinar: Meet the new Talos 12 TEM - 18th Sept<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Message-ID:<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> <<a href="mailto:SJ0P222MB0186DA39D31060E29F9C477A860FA@SJ0P222MB0186.NAMP222.PROD.OUTLOOK.COM">SJ0P222MB0186DA39D31060E29F9C477A860FA@SJ0P222MB0186.NAMP222.PROD.OUTLOOK.COM</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> <o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Content-Type: text/plain; charset="utf-8"<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Hi,<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> Pleased to share details of our latest webinar:<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Meet the new Talos 12 TEM<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">18 September 2025<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Register here: <a href="https://urldefense.com/v3/__https:/www.thermofisher.com/uk/en/home/events/meet-the-new-talos-12-tem.html__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhkyK7dGo$">https://urldefense.com/v3/__https://www.thermofisher.com/uk/en/home/events/meet-the-new-talos-12-tem.html__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhkyK7dGo$</a><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Session 1: September 18, 10 a.m. CST/BJT, 11 a.m. JST, 12 p.m. AEST<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Session 2: September 18, 2 p.m. CEST, 8 a.m. EDT<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Session 3: September 18, 2 p.m. EDT, 11 a.m. PDT<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">The user-friendly Thermo Scientific Talos 12 TEM offers exceptional performance for a diverse range of scientific imaging and experimentation needs. It features a reduced footprint, a robust enclosure, and systems that improve accessibility, bringing trusted performance and automation to more researchers and their labs.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">In this webinar, you will learn about:<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> * Recent advancements in 120 kV TEM hardware and software that improve accessibility and automation<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> * How built-in automation enables high-throughput, unattended batch acquisition of multi-scale data<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> * New workflows enhanced with AI-training algorithms designed to accelerate and automate high-throughput acquisition and analysis of nanoparticle data under cryogenic conditions<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Join us to explore how these advancements make traditional TEM technology more accessible, efficient, and powerful for a wider range of users in life sciences and clinical research.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">About the speakers<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Tim Booth, Product Marketing Manager, Thermo Fisher Scientific<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">With 23 years of experience in cryo-EM, Tim?s expertise encompasses structural biology, workflow strategy, and core facility management. In his current role, he is responsible for promoting and supporting accessible solutions that lower barriers for those new to TEM and cryo-EM and provide the most straightforward and economical approach to reaching higher data quality and structural resolutions.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Ond?ej Vyroubal, Engineer, Thermo Fisher Scientific<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Ond?ej holds a master's degree in physics engineering and nanotechnology from Brno University of Technology. He has a strong background in R&D with eight years at Thermo Fisher Scientific, where he helped develop advanced cameras and detectors and now leads the technical architecture of the Talos 12 TEM.?<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Reint Boer Iwema, Software Product Manager, Thermo Fisher Scientific<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">After obtaining?his master's degree in chemistry, Reint developed cryo-EM and X-ray crystallography software at Imperial College London and Leiden University. He previously worked on medical imaging software and currently leads software development for the cryo-electron tomography workflow on FIB-SEM and TEM?systems.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Look forward to seeing some of you there,<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Claire<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Claire Naylor, Ph.D (she/her/hers)<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Regional Market Development EMEA<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Thermo Fisher Scientific<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Stafford House, 1 Boundary Park, Hemel Hempstead Industrial Estate | HP2 7GE | UK<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><a href="mailto:Claire.naylor@thermofisher.com%3cmailto:Claire.naylor@thermofisher.com">Claire.naylor@thermofisher.com<mailto:Claire.naylor@thermofisher.com</a>> | <a href="https://urldefense.com/v3/__http:/www.thermofisher.com__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhSYImygg$">https://urldefense.com/v3/__http://www.thermofisher.com__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhSYImygg$</a><<a href="https://urldefense.com/v3/__http:/www.thermofisher.com/__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhh5rHkrc$">https://urldefense.com/v3/__http://www.thermofisher.com/__;!!Mih3wA!AciF3_05q5EXhtgqGWNtCEZU_luTD7f4KQdS9p1ecePK7i5Ezcbtl1VslVH9JsIlbiH8BTxOkTmCI7dgIEDEqlEhh5rHkrc$</a>> | Mobile: +44 (0)7353 153679<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">URL: <<a href="https://urldefense.com/v3/__http:/mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20250909/e6ca2130/attachment-0001.html__;!!G8Er-fQLpA!JnBXJdzYclzZzGvIsPdOhMvs-dmJ8njYpfbOwlBeyhgaIlTWei80ydJlQ7d4NC-k-LJX8jJLge-pRGdFjxaPXKN-Iu7jVDhRiOqMyFos85E$">https://urldefense.com/v3/__http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20250909/e6ca2130/attachment-0001.html__;!!G8Er-fQLpA!JnBXJdzYclzZzGvIsPdOhMvs-dmJ8njYpfbOwlBeyhgaIlTWei80ydJlQ7d4NC-k-LJX8jJLge-pRGdFjxaPXKN-Iu7jVDhRiOqMyFos85E$</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">------------------------------<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Message: 3<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Date: Tue, 9 Sep 2025 15:55:51 +0000<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">From: "Thomas, Leonard M." <<a href="mailto:lmthomas@ou.edu">lmthomas@ou.edu</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">To: "'3dem@ncmir.ucsd.edu'" <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Subject: [3dem] Ethane Cylinder Age<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Message-ID:<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> <<a href="mailto:SA1PR03MB634074F9B5B4B609D318D9E0B70FA@SA1PR03MB6340.namprd03.prod.outlook.com">SA1PR03MB634074F9B5B4B609D318D9E0B70FA@SA1PR03MB6340.namprd03.prod.outlook.com</a>><o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"> <o:p></o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Hello All,<o:p></o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Sort of oddish question but how long is a research grade Ethane cylinder good for? We have one that is probably at least 6 years old and I was wondering when I should worry about the quality of the ethane for plunge freezing. It really was not used much when it was first purchased but has been since we got a hold of it.<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Thank in advance for any thoughts,<o:p></o:p></span></pre>
<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Len<o:p></o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Leonard M. Thomas, Ph.D.<o:p></o:p></span></pre>
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<pre><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Biomolecular Structure Core Director<o:p></o:p></span></pre>
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