[3dem] Experience with Simple Origin Cryo Holders (Model 201)?

Mon Nov 10 23:58:45 PST 2025

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--

Thomas Boesen

Senior Researcher, PhD
Head of Danish National cryo-EM Facility (Aarhus node) - EMBION

Interdiciplinary Nanoscience Center - iNANO

Department of Molecular Biology and Genetics - MBG

Center for Electromicrobiology - CEM
Nordic EMBL Partnership for Molecular Medicine - DANDRITE
Aarhus University
Gustav Wieds Vej 14
8000 Aarhus C
Denmark

Mobile: +45 2899 7699<tel:+4528997699>

Krios1: +45 871 55263<tel:+4587155263> (Bldg. 1524 room 021A)

Krios2: +45 871 56598<tel:+4587156598> (Bldg. 1524 room 023)

Tecnai1: +45 871 56791<tel:+4587156791> (Bldg. 1590 room 055B)

Offices: Bldg. 1872 room 478 (MBG) and Bldg. 1590 room 146 (iNANO)
Labs: Bldg. 1872 room 473 (MBG) and Bldg. 1590 room 067 (iNANO)

E-mail: thb at inano.au.dk<mailto:thb at inano.au.dk>

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From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Tobias Furstenhaupt via 3dem <3dem at ncmir.ucsd.edu>
Date: Tuesday, 11 November 2025 at 08.47
To: Berith Isaac <bisaac at brandeis.edu>
Cc: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Experience with Simple Origin Cryo Holders (Model 201)?

Hi Berith,

we are very happy with our Simple Origin Holder. We have the one that holds 2 autogrids. We had two problems related to the holder, but Simple Origin fixed them on their own costs without even asking when we bought the holder or mentioning the word 'warrenty'.

We also have two Gatan 626 holder (10++ years each) each for one bare grid, so I naturally compare the SO holder with those: inserting the grids/autogrids into the holder is easier and more secure on the SO holder. Gatan holds the temperature for 2-4 hours while the SO holds it for 8-10 hours. With the SO we are now able to do single particle analysis over night (on our side-entry TEM). The SO holder clamps down the grids with metal-flaps that might break after some years. But from own experience I can say that replacing this part is easy to do but needs some adjustment and removing of material with very fine sandpaper. But I was able to do it in 2 hours. Our Gatan 626 uses a clip-ring that is (especially under liquid nitrogen) a bit fiddly and over time those clips wear down and clamp worse and worse and needs to be replaced - normally we realise that it is time to replace the clip if we unsuccessfully tried to clap the grid for more than 30 minutes or more (fortunately for those cases we _always_ keep a brand-new clip in storage). This happens for us every 3 years.
My preference is the SO clamping mechanism as it is easier when dealing with grids under nitrogen.

The loading-station of the SO is better than the one from the Gatan 626: the SO station has light build into the chamber so that visibility is much better. It also has a small heater built in that can be switched on to evaporate more liqid nitrogen to have more cold protective nitrogen gas produced to protect from humidity contamination while handling the grid.
Last not least the vacuum-connection to the dewar of the SO holder is bigger in diameter (ie you can connect larger diameter tubes) than on the Gatan 626 - which should improve pumping times and end-vacuum in the dewar.

Before buying the SO holder, we tested the SO holder and the ELSA holder and the SO convinced us more. And it is a small company so communication is easier and they take you seriously as a customer. Although we had two technical problems with our holder, the company was very responsive and helpful in fixing it on their costs. We never regretted buying the SO holder and it is doing a reliable job with our cryo samples.

If you have any questions or want fotos/videos, please let us know :)

cheers
Tobias

-----------------------------------
Tobias Fürstenhaupt, PhD
Electron Microscopy Facility
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstrasse 108
01307 Dresden, Germany

phone: (+49) (0)351 / 210-2690
cell: (+49) (0)176 / 44498706

----- Original Message -----
> From: "Berith Isaac via 3dem" <3dem at ncmir.ucsd.edu>
> To: 3dem at ncmir.ucsd.edu
> Sent: Monday, 10 November, 2025 16:53:36
> Subject: [3dem] Experience with Simple Origin Cryo Holders (Model 201)?

> Hi everyone,
>
> Does anyone have experience with the Simple Origin cryo holders? I’m
> particularly interested in the Model 201, which can accommodate both
> standard and clipped (Autogrid) grids, and how it compares in practice to
> the Gatan ELSA cryo holder. We’re exploring options for efficiently
> transferring grids between our Tundra and a side-entry cryo-TEM for
> cryo-tomography work.
>
> Thanks very much for any insights or recommendations!
>
> Best,
> Berith
>
> --
> Berith Isaac
> Manager
> Brandeis Electron Microscopy Facility
>
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