[3dem] Positively charged carbon grids

[Benjamin Apker]

Dear David,

As mentioned by the others, a positive charge can be achieved either by
discharging in a plasma with an amylamine atmosphere or post-discharge
treatment of the grid.

A quick note that the amylamine atmosphere will contaminate/coat the
surfaces of your glow discharge system for future discharges. The GloQube
Plus Dual-chamber System (system page here
<https://urldefense.com/v3/__https://www.mitegen.com/product/glow-discharge-system-for-tem-grids/__;!!Mih3wA!FBrH2ZBiX5cmFjBrqijbiMRH8ZSVHf1bFeipwQ4ml1GDsLNy0aqyA4gNX9CWGQqU-l4H7Fxsi75_80hS-z0lV8YwmfT-$ >) has
separate chambers to accommodate alternative discharge atmospheres and
avoid cross-contamination.

There was an informative discussion on this topic (and whether it results
in a hydrophilic or hydrophobic surface) on this forum back in 2009 (thread
here <https://mail.ncmir.ucsd.edu/pipermail/3dem/2009-February/001505.html >
).

Cheers,
Ben

On Mon, May 19, 2025 at 7:45 AM PEREZ-MORGA David via 3dem <
3dem at ncmir.ucsd.edu> wrote:

> Dear 3dem users,
> Any advice on how to posively charge  TEM carbon-film grids?
> Thanks
> David
>
> David Pérez-Morga
>
>
>
> *Molecular Parasitology Lab.
> <https://urldefense.com/v3/__http://parasmol.ulb.be/__;!!Mih3wA!GCidJgEO8IR5DBtmsHlZ1xMn5tStEEgMF3x2YYQr6hsFcnpO4LeTFOMnSCWWcR_etu3hIvvHCr2PjsVOmOUFMk5JlLkK6g$>**
> <https://urldefense.com/v3/__http://parasmol.ulb.be/__;!!Mih3wA!GCidJgEO8IR5DBtmsHlZ1xMn5tStEEgMF3x2YYQr6hsFcnpO4LeTFOMnSCWWcR_etu3hIvvHCr2PjsVOmOUFMk5JlLkK6g$>*
>     IBMM-Université Libre de Bruxelles
>
> *Electron Microscopy Lab.
> <https://urldefense.com/v3/__https://www.cmmi.be/microscopy__;!!Mih3wA!GCidJgEO8IR5DBtmsHlZ1xMn5tStEEgMF3x2YYQr6hsFcnpO4LeTFOMnSCWWcR_etu3hIvvHCr2PjsVOmOUFMk5KQGcYng$>*
>    CMMI-Université Libre de Bruxelles
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem 
>


-- 
Benjamin Apker
Chief Executive Officer
MiTeGen
Expanding Tools for Cryo-EM:
https://urldefense.com/v3/__https://www.mitegen.com/product-category/cryo-em/__;!!Mih3wA!FBrH2ZBiX5cmFjBrqijbiMRH8ZSVHf1bFeipwQ4ml1GDsLNy0aqyA4gNX9CWGQqU-l4H7Fxsi75_80hS-z0lVyLn4MYX$ 
<https://urldefense.com/v3/__http://www.mitegen.com/cryo-em__;!!Mih3wA!FBrH2ZBiX5cmFjBrqijbiMRH8ZSVHf1bFeipwQ4ml1GDsLNy0aqyA4gNX9CWGQqU-l4H7Fxsi75_80hS-z0lV5i21zXF$ >

95 Brown Road
MS 1034, Suite 124
Ithaca, NY 14850
office: +1-607-266-8877
fax:    +1-607-697-0400
___________________________________________________________
This message contains confidential information and is intended only for the
individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system. E-mail transmission cannot be
guaranteed to be secure or error-free as information could be intercepted,
corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
The sender therefore does not accept liability for any errors or omissions
in the contents of this message, which arise as a result of e-mail
transmission. MiTeGen, 95 Brown Road, Suite 124, Ithaca, NY 14850.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20250519/09424c5a/attachment.html>