[3dem] Alkylamine vapor while glow discharge
Eduardo Sanz Garcia
esanzgar at gmail.com
Thu Feb 5 18:46:51 PST 2009
The use of alkylamine vapors in glow discharge apparatus makes the
grid positively charged. But I found some contradictory information
about the hydrophobicity:
Some say that it makes the surface hydrophilic, others hydrophobic.
If it makes the surface hydrophobic, while is still used in cryoEM?
Shouldn't that prevent the fluid from spreading evenly on the grid
surface?
Does someone have experience in this type of treatment?
Additionally,
Has someone use magnesium acetate, lysine or polylysine after glow
discharge to make the surface positively charged?
Thank you.
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