[3dem] 3rd Grenoble Practical Workshop on Helical Symmetry determination and reconstruction

[Leandro F. Estrozi]

*Dear EM (Helical Assemblies Interested) Community,*

We are pleased to announce the third edition of the Grenoble practical 
workshop focused on the determination of helical symmetry in biological 
polymers imaged by cryo-EM. The workshop will take place *online from 
July 21st to 25th, 2025*.

Please note that due to specific procedural requirements, *projects 
involving amyloids or microtubules will not be accepted*.

As in previous editions, we are honored to welcome two distinguished 
speakers: *Ed Egelman* and *Arjen Jakobi*, whose expertise will greatly 
enrich both our discussions and hands-on sessions.

Determining helical parameters continues to be a major challenge for 
many researchers. This workshop is designed to provide *practical, 
hands-on guidance* through the complexities of this process. Topics will 
include interpreting power spectra, analyzing 2D class averages, and 
examining raw data to extract meaningful structural insights.

While *Helixplorer* (https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!E8k6u4PapE61RoHy1fNa_JmGof9e65JNpZs2Dcf7d1ogbvN9gxduVMX-fpPxMI-nzq_qTqbseDr52Jf6kB_Jv66N9Rjr$ ) will play a 
central role in our practical sessions, we also encourage participants 
to explore alternative approaches to address a variety of research needs.

Beyond helical parameter determination, we will also explore *broader 
reconstruction strategies*, including managing flexible helices, working 
with multiple symmetries, and integrating data from different filament 
symmetries using subtraction techniques.

We welcome applicants at all career stages—PhD students, postdocs, 
senior scientists, and beyond—*who are currently working on helical 
samples and facing structural analysis challenges*. During the workshop, 
participants will use their own data, sharing selected raw images, 2D 
class averages, and power spectra with the organizers. The specific 
identity and function of your protein or filament will remain confidential.

To apply, please email your application—including a brief description of 
your project and the difficulties you’re encountering, as well as 
screenshots of your raw data and 2D class averages with the 
corresponding *pixel size*—to:

  *

    *ambroise.desfosses at ibs.fr*

  *

    *leandro.estrozi at ibs.fr*

*Application deadline: June 10th.*

We look forward to another engaging and fruitful workshop and hope to 
see you online in July!

*Warm regards,
*
/Ambroise & Leandro/
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