[3dem] Postdoctoral position in Phage Lysis at eBIC, Diamond

[Savva, Christos (DLSLtd,RAL,LSCI)]

Dear colleagues

We have an exciting opportunity for a Postdoctoral Research Associate to join the electron Bio-Imaging Centre (eBIC) at Diamond Light Source to study the structural basis of bacteriophage host-cell lysis. The successful candidate will use high resolution Cryo-Tomography as well as single particle Cryo-EM to unravel the molecular mechanisms by which bacteriophages escape from the host cell. The position is for a fixed period of 3 years.

The project is a collaboration between the group of Dr. Christos Savva at eBIC and Prof. Ryland Young at Texas A&M University. eBIC is the UK National Centre for cryo-electron microscopy in the life sciences. eBIC provides scientists with state-of-the-art equipment and expertise in the field of cryo-electron microscopy (CryoEM), for single particle analysis, cryo-electron tomography and electron crystallography. Together with eBIC for Industry, our site hosts eight TEM microscopes for the life sciences (5 Titan Krios's, 1 Talos Arctica, 2 Glacios's), 2 Dual Beam SEM’s capable of lamella milling (Scios and Aquilos 2 with cryo lift out) and a Leica cryoCLEM.

For previous work please see:
Spatial and temporal control of lysis by the <https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/38126784/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtDqrwPbhg$ > lambda<https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/38126784/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtDqrwPbhg$ > holin.<https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/38126784/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtDqrwPbhg$ >
Stable micron-scale holes are a general feature of canonical holins.<https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/24164554/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtBbLK7fRA$ >
Micron-scale holes terminate the phage infection cycle.<https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/20080651/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtC5pnfWNw$ >
The holin of bacteriophage <https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/18788120/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtDjIWWH1w$ > lambda<https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/18788120/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtDjIWWH1w$ > forms rings with large diameter.<https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/18788120/__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtDjIWWH1w$ >


About You
You will hold or be close to completing a PhD in life sciences, with strong experience in structural biology (Cryo-EM, SPA and/or Tomography) and expertise in bacterial expression systems and protein purification. Experience in microbiology/phage biology will be considered an advantage but not essential.

For informal enquiries feel free to contact Christos Savva (christos.savva at diamond.ac.uk<mailto:christos.savva at diamond.ac.uk>)

The Application deadline is July 13th 2025. For full details of the position and to apply please visit:

https://urldefense.com/v3/__https://vacancies.diamond.ac.uk/vacancy/pdra-structural-basis-of-bacteriophage-host-cell-lysis-588077.html__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtCs0IF1cg$ 


 Best wishes

Christos Savva
Principal EM Scientist

Office Tel: +44 1235 778 976

[signature_3278397444]
electron Bio-Imaging Centre (eBIC)
Diamond Light Source Ltd
Diamond House
Harwell Science and Innovation Campus
Didcot Oxfordshire OX11 0DE

eBIC website<https://urldefense.com/v3/__https://www.diamond.ac.uk/Instruments/Biological-Cryo-Imaging/eBIC.html__;!!Mih3wA!F0ipUvmM_FrqtXhNmpo7hwfbPhFZ2wtKKTrgJZUKjCIVM24f-xgA4ezjRQsMe2Jetufy1AOrFDLfOvjERM-FmmoBTtCCAlmvIA$ >
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