[3dem] Seeking Advice on BSL-2 CryoEM Laboratory Setup and Safety Protocols

[Jason NG (CPOS)]

Dear all,

Thank you very much for your replies to my email and to those who messaged me privately regarding the BSL-2 lab setup. I sincerely appreciate the detailed information and experiences you have shared-they are extremely helpful and valuable.

I am currently summarizing all your comments and suggestions to present to my senior. Please feel free to continue the discussion and share any further opinions or advice you may have.

Regards,
Jason

From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Jason NG (CPOS) via 3dem
Sent: 12 June 2025 17:00
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Seeking Advice on BSL-2 CryoEM Laboratory Setup and Safety Protocols

Dear CryoEM Community,

We are currently evaluating the risks of becoming BSL-2 CryoEM laboratory and would greatly appreciate your guidance on several safety and setup considerations.

Specifically, we are interested in understanding:

  *   Given that the electron beam can potentially generate aerosols or cause warming within TEMs, what disinfection protocols or measures have you found effective for maintaining biosafety?
  *   Is implementing negative room pressure necessary in a CryoEM lab at BSL-2?
  *   For sample preparation instruments such as the TFS Vitrobot and Leica GP2, is it recommended to place them inside a biosafety cabinet? How about large instruments like the Leica ICE-are there specific safety measures we should consider?

Your insights and experiences would be extremely helpful as we evaluate the best practices for creating a safe and effective CryoEM environment. Thank you very much for your time and guidance.

Best Regards,
Jason NG
Senior Technical Assistant
LKS Cryo-EM Laboratory

[cid:image001.jpg at 01DBDED6.C90BC140]

Centre for PanorOmic Sciences
LLG03, Laboratory Block
21 Sassoon Road, Pokfulam, Hong Kong
P: +852 3910-3528
E: jasonng.cpos at hku.hk<mailto:jasonng.cpos at hku.hk>
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