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<p class="MsoNormal"><span style="font-size:11.0pt">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Thank you very much for your replies to my email and to those who messaged me privately regarding the BSL-2 lab setup. I sincerely appreciate the detailed information and experiences you have shared—they are
extremely helpful and valuable.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">I am currently summarizing all your comments and suggestions to present to my senior. Please feel free to continue the discussion and share any further opinions or advice you may have.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Jason<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0cm 0cm 0cm">
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;mso-ligatures:none">From:</span></b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif;mso-ligatures:none"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Jason NG (CPOS) via 3dem<br>
<b>Sent:</b> 12 June 2025 17:00<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Seeking Advice on BSL-2 CryoEM Laboratory Setup and Safety Protocols<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Dear CryoEM Community,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">We are currently evaluating the risks of becoming BSL-2 CryoEM laboratory and would greatly appreciate your guidance on several safety and setup considerations.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Specifically, we are interested in understanding:<o:p></o:p></span></p>
<ul style="margin-top:0cm" type="disc">
<li class="MsoNormal" style="mso-list:l1 level1 lfo3"><span style="font-size:11.0pt">Given that the electron beam can potentially generate aerosols or cause warming within TEMs, what disinfection protocols or measures have you found effective for maintaining
biosafety?<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo3"><span style="font-size:11.0pt">Is implementing negative room pressure necessary in a CryoEM lab at BSL-2?<o:p></o:p></span></li><li class="MsoNormal" style="mso-list:l1 level1 lfo3"><span style="font-size:11.0pt">For sample preparation instruments such as the TFS Vitrobot and Leica GP2, is it recommended to place them inside a biosafety cabinet? How about large instruments like the
Leica ICE—are there specific safety measures we should consider?<o:p></o:p></span></li></ul>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Your insights and experiences would be extremely helpful as we evaluate the best practices for creating a safe and effective CryoEM environment. Thank you very much for your time and guidance.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">Best Regards,<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">Jason NG<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#548DD4">Senior Technical Assistant<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#548DD4">LKS Cryo-EM Laboratory</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="color:black;mso-ligatures:none"><img width="292" height="41" style="width:3.0416in;height:.427in" id="Picture_x0020_12" src="cid:image001.jpg@01DBDED6.C90BC140"></span><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F4E79">Centre for PanorOmic Sciences</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">LLG03, Laboratory Block<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">21 Sassoon Road, Pokfulam, Hong Kong<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">P: +852 3910-3528</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">E: </span><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F4E79"><a href="mailto:jasonng.cpos@hku.hk">jasonng.cpos@hku.hk</a><o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#1F497D">W: </span><span style="color:#1F4E79"><a href="https://urldefense.com/v3/__https:/cpos.hku.hk/__;!!Mih3wA!B5co-igM_UHRmCF1lSC-8fHY1eebmRgfFqxjKo2KEWhA_VnVBHdVH1ZLRO9f_JY6lLds5D1C_SANjg3mKpVKQaHmXg$" target="_blank"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:blue">https://cpos.hku.hk</span></a></span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal"><a href="https://urldefense.com/v3/__http:/www.facebook.com/cposhku__;!!Mih3wA!B5co-igM_UHRmCF1lSC-8fHY1eebmRgfFqxjKo2KEWhA_VnVBHdVH1ZLRO9f_JY6lLds5D1C_SANjg3mKpXC2V-dIQ$"><span lang="EN-US" style="font-family:"Arial",sans-serif;color:#1F497D;mso-ligatures:none;text-decoration:none"><img border="0" width="50" height="50" style="width:.5208in;height:.5208in" id="Picture_x0020_6" src="cid:image002.png@01DBDED6.C90BC140" alt="A blue circle with a white letter f in it
Description automatically generated"></span></a><span lang="EN-US" style="font-family:"Arial",sans-serif;color:#1F497D"> </span><a href="https://urldefense.com/v3/__https:/www.instagram.com/hkucpos__;!!Mih3wA!B5co-igM_UHRmCF1lSC-8fHY1eebmRgfFqxjKo2KEWhA_VnVBHdVH1ZLRO9f_JY6lLds5D1C_SANjg3mKpX6V0tH6g$"><span lang="EN-US" style="font-family:"Arial",sans-serif;color:#1F497D;mso-ligatures:none;text-decoration:none"><img border="0" width="50" height="50" style="width:.5208in;height:.5208in" id="Picture_x0020_7" src="cid:image003.png@01DBDED6.C90BC140" alt="A logo of a camera
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</span><a href="https://urldefense.com/v3/__https:/twitter.com/hkucpos__;!!Mih3wA!B5co-igM_UHRmCF1lSC-8fHY1eebmRgfFqxjKo2KEWhA_VnVBHdVH1ZLRO9f_JY6lLds5D1C_SANjg3mKpXWoJl-nQ$"><span style="color:#1F4E79;mso-ligatures:none;text-decoration:none"><img border="0" width="49" height="49" style="width:.5104in;height:.5104in" id="Picture_x0020_8" src="cid:image004.png@01DBDED6.C90BC140" alt="A white x in a black circle
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