[3dem] Use of methanol in cryo-EM samples
Ruben Diaz Avalos
rdiaz at lji.org
Wed May 8 10:59:59 PDT 2024
Hi Jie,
I have used up to 20% methanol (and ethanol, acetonitrile, acetone...) in
some samples; the amount of solvent in the frozen samples is so minimal
that there is no way they can cause any damage to anything, but I can tell
you that the beam-induced movement of these samples is much worse than in a
purely aqueous buffer.
Ruben.
On Wed, May 8, 2024 at 9:44 AM Jie Zhang <jiezhang518 at utexas.edu> wrote:
> Hi everyone,
> I am trying to use methanol in my cryo-EM samples (specifically for
> Glacios and Krios). Is there anyone who has prepared the grids with samples
> containing more than 10& methanol? I just want to make sure high
> concentration of methanol is okay for these machines.
> Thank you.
>
> Best regards,
> Jie Zhang
>
>
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--
**********************************************
Ruben Diaz Avalos,
La Jolla Institute for Immunology,
9420 Athena Circle Dr.,
La Jolla, CA 92037
(858)752-6960
**********************************************
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