[3dem] Use of methanol in cryo-EM samples

[Ruben Diaz Avalos]

Hi Jie,

I have used up to 20% methanol (and ethanol, acetonitrile, acetone...) in
some samples; the amount of solvent in the frozen samples is so minimal
that there is no way they can cause any damage to anything, but I can tell
you that the beam-induced movement of these samples is much worse than in a
purely aqueous buffer.

Ruben.

On Wed, May 8, 2024 at 9:44 AM Jie Zhang <jiezhang518 at utexas.edu> wrote:

> Hi everyone,
> I am trying to use methanol in my cryo-EM samples (specifically for
> Glacios and Krios). Is there anyone who has prepared the grids with samples
> containing more than 10& methanol? I just want to make sure high
> concentration of methanol is okay for these machines.
> Thank you.
>
> Best regards,
> Jie Zhang
>
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>


-- 
**********************************************
Ruben Diaz Avalos,
La Jolla Institute for Immunology,
9420 Athena Circle Dr.,
La Jolla, CA 92037
(858)752-6960
**********************************************
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20240508/897d6f9b/attachment-0001.html>