<div dir="ltr"><div class="gmail_default" style="font-family:garamond,times new roman,serif">Hi Jie,</div><div class="gmail_default" style="font-family:garamond,times new roman,serif"><br></div><div class="gmail_default" style="font-family:garamond,times new roman,serif">I have used up to 20% methanol (and ethanol, acetonitrile, acetone...) in some samples; the amount of solvent in the frozen samples is so minimal that there is no way they can cause any damage to anything, but I can tell you that the beam-induced movement of these samples is much worse than in a purely aqueous buffer.</div><div class="gmail_default" style="font-family:garamond,times new roman,serif"><br></div><div class="gmail_default" style="font-family:garamond,times new roman,serif">Ruben.</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, May 8, 2024 at 9:44 AM Jie Zhang <<a href="mailto:jiezhang518@utexas.edu">jiezhang518@utexas.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Hi everyone,<br>
I am trying to use methanol in my cryo-EM samples (specifically for Glacios and Krios). Is there anyone who has prepared the grids with samples containing more than 10& methanol? I just want to make sure high concentration of methanol is okay for these machines.<br>
Thank you.<br>
<br>
Best regards,<br>
Jie Zhang <br>
<br>
<br>
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</blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature"><div dir="ltr"><div>**********************************************</div>Ruben Diaz Avalos,<div>La Jolla Institute for Immunology,</div><div>9420 Athena Circle Dr.,</div><div>La Jolla, CA 92037</div><div>(858)752-6960</div><div>**********************************************</div></div></div>