[3dem] 2nd Grenoble Practical Workshop on Helical Symmetry determination and reconstruction

[Leandro F. Estrozi]

Dear EM (helical assemblies interested) community,


We are delighted to announce the second edition of the Grenoble 
Practical Workshop dedicated to helical symmetry determination of 
biological polymers imaged by cryo-EM, scheduled for July *8th to 12th, 
2024, online*.

Building upon the success of last year's workshop, where we had over 20 
projects, more than half of which were resolved during the workshop or 
shortly after, we are excited to continue facilitating breakthroughs in 
helical structure determination.

Please note that this year, due to the specific procedures required, we 
regret to inform you that projects involving *amyloid* *or* 
*microtubules will not be accepted*.

We are honored to host two distinguished speakers this year: *Ed 
Egelman* and *Arjen Jakobi*, who will enrich our discussions and 
practical sessions with their expertise.

Determining the helical parameters remains a significant challenge for 
many researchers, and our workshop aims to provide hands-on guidance 
through this intricate process. We will delve into power spectra 
interpretation, 2D class-averages analysis, and raw data inspection to 
extract valuable insights.

While helixplorer (https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!HpHyFMhwk_z8WWm7S1anqKBZZSu0QnZgyGSyJLUkQAcxFOW4a89Iu21P3iMIC6kIe-EN8nXWfhPedMK3kdpy1Qv5c5rk$ ) will be a key tool 
covered in the practical sessions, we encourage exploration beyond this 
approach to accommodate diverse research needs.

In addition to helical parameters determination, we will address broader 
reconstruction strategies, such as handling flexible helices, navigating 
multiple symmetries, and integrating data from different symmetry 
filaments through subtraction approaches.

Applicants at any career level (PhD, postdocs, senior scientists, etc.) 
are welcome to apply, provided they have an ongoing project involving a 
helical sample and are encountering challenges in solving its structure. 
Participants will work on their own data during the workshop, sharing 
selected raw images, 2D class-averages, and power spectra with the 
organizers while maintaining confidentiality regarding the specific 
nature and function of their protein/filaments.

To apply, please send your application, including a brief description of 
your project and difficulties, along with screenshots of raw data and 2D 
class-averages, to:

ambroise.desfosses at ibs.fr

leandro.estrozi at ibs.fr

*The deadline for applications is June 15th*.

We are eagerly anticipating another productive workshop, and we look 
forward to welcoming you to the virtual platform.

Warm regards,

Ambroise & Leandro
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