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Dear EM (helical assemblies interested) community,
<p> <br>
We are delighted to announce the second edition of the Grenoble
Practical Workshop dedicated to helical symmetry determination of
biological polymers imaged by cryo-EM, scheduled for July <b>8th
to 12th, 2024, online</b>.<br>
<br>
Building upon the success of last year's workshop, where we had
over 20 projects, more than half of which were resolved during the
workshop or shortly after, we are excited to continue facilitating
breakthroughs in helical structure determination.<br>
<br>
Please note that this year, due to the specific procedures
required, we regret to inform you that projects involving <b>amyloid</b>
<b>or</b> <b>microtubules will not be accepted</b>.<br>
<br>
We are honored to host two distinguished speakers this year: <b>Ed
Egelman</b> and <b>Arjen Jakobi</b>, who will enrich our
discussions and practical sessions with their expertise.<br>
<br>
Determining the helical parameters remains a significant challenge
for many researchers, and our workshop aims to provide hands-on
guidance through this intricate process. We will delve into power
spectra interpretation, 2D class-averages analysis, and raw data
inspection to extract valuable insights.<br>
<br>
While helixplorer (<a class="moz-txt-link-freetext" href="https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!HpHyFMhwk_z8WWm7S1anqKBZZSu0QnZgyGSyJLUkQAcxFOW4a89Iu21P3iMIC6kIe-EN8nXWfhPedMK3kdpy1Qv5c5rk$" moz-do-not-send="true">https://rico.ibs.fr/helixplorer/</a>)
will be a key tool covered in the practical sessions, we encourage
exploration beyond this approach to accommodate diverse research
needs.<br>
<br>
In addition to helical parameters determination, we will address
broader reconstruction strategies, such as handling flexible
helices, navigating multiple symmetries, and integrating data from
different symmetry filaments through subtraction approaches.<br>
<br>
Applicants at any career level (PhD, postdocs, senior scientists,
etc.) are welcome to apply, provided they have an ongoing project
involving a helical sample and are encountering challenges in
solving its structure. Participants will work on their own data
during the workshop, sharing selected raw images, 2D
class-averages, and power spectra with the organizers while
maintaining confidentiality regarding the specific nature and
function of their protein/filaments.<br>
<br>
To apply, please send your application, including a brief
description of your project and difficulties, along with
screenshots of raw data and 2D class-averages, to:<br>
<br>
<a class="moz-txt-link-abbreviated moz-txt-link-freetext"
href="mailto:ambroise.desfosses@ibs.fr" moz-do-not-send="true">ambroise.desfosses@ibs.fr</a><br>
<br>
<a class="moz-txt-link-abbreviated moz-txt-link-freetext"
href="mailto:leandro.estrozi@ibs.fr" moz-do-not-send="true">leandro.estrozi@ibs.fr</a><br>
<br>
<b>The deadline for applications is June 15th</b>.<br>
<br>
We are eagerly anticipating another productive workshop, and we
look forward to welcoming you to the virtual platform.<br>
<br>
Warm regards,<br>
<br>
Ambroise & Leandro</p>
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