[3dem] Fall 2024 Tomography and Cryo-FIB Workshop at UW-Madison — September 23-27, 2024

[CORINA ELIZABETH EVANS]

Hi Everyone,

We are thrilled to announce the Fall 2024 Tomography and Cryo-FIB Workshop hosted by the Midwest Center for Cryo-Electron Tomography (MCCET) at UW-Madison. The workshop will be held from September 23-27, 2024. In addition to our MCCET team, we have an exciting group of external/speakers that includes Claudia Lopez (Pacific Northwest Cryo-EM Center), Zunlong Ke (UT-Austin), Lauren Ann Metskas (Purdue), Matt Swulius (Penn State – Hershey), Louise Bertrand (Leica), Haridas Pudavar (Leica), Sergey Suslov (TFS), and Zoltan Metlagel(TFS). Other speakers to be confirmed!

Training topics will include:

  *   Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters.
  *   Training in data acquisition workflows that include screening, data collection, and flexible scripting.
  *   Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging.
  *   Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2.
  *   Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods.

The morning theory sessions of the workshop will be hybrid and the afternoon practical sessions will be in-person only.

Registration: In-person space is limited, so we encourage you to apply at your earliest convenience. For more details and registration, please visit our workshop page<https://urldefense.com/v3/__https://explore.wisc.edu/LP=2218__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73-kYXMM-A$ > –  Registration closes August 1st, 2024.

Questions:  For inquiries regarding meeting content, registration, and local lodging, please direct your questions to the following three contacts at Email Us<mailto:cotten at wisc.edu;ceevans3 at wisc.edu;cryoem at biochem.wisc.edue>:
Alex Cotten (cotten at wisc.edu<mailto:cotten at wisc.edu>)
Corina Elizabeth Evans (ceevans3 at wisc.edu<mailto:ceevans3 at wisc.edu>)
UW-Madison Cryo-EM (cryoem at biochem.wisc.edu<mailto:cryoem at biochem.wisc.edu>).

We look forward to welcoming you to this enriching event!



Corina Elizabeth Evans
Administrative Assistant II
D: (608) 262-7175
E: ceevans3 at wisc.edu<mailto:ceevans3 at wisc.edu>
E: cryoem at biochem.wisc.edu<mailto:cryoem at biochem.wisc.edu> | https://urldefense.com/v3/__https://cryoem.wisc.edu/__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73_qh8QXHA$ 

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