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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#212121">Hi Everyone,</span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span style="font-family:"Arial",sans-serif;color:#212121">We are thrilled to announce the Fall 2024 Tomography and Cryo-FIB Workshop hosted by the Midwest Center for Cryo-Electron Tomography (MCCET) at UW-Madison. The workshop will be held from September 23-27,
2024. In addition to our MCCET team, we have an exciting group of external/speakers that includes Claudia Lopez (Pacific Northwest Cryo-EM Center),<span class="apple-converted-space"> </span></span><span class="spelle"><span style="color:#212121">Zunlong</span></span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif;color:#212121"> </span></span><span style="font-family:"Arial",sans-serif;color:#212121">Ke
(UT-Austin), Lauren Ann<span class="apple-converted-space"> </span></span><span class="spelle"><span style="color:#212121">Metskas</span></span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif;color:#212121"> </span></span><span style="font-family:"Arial",sans-serif;color:#212121">(Purdue),<span class="apple-converted-space"> Matt </span></span><span class="spelle"><span style="color:#212121">Swulius</span></span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif;color:#212121"> (Penn
State – Hershey), </span></span><span style="font-family:"Arial",sans-serif;color:#212121">Louise Bertrand (Leica), Haridas<span class="apple-converted-space"> </span></span><span class="spelle"><span style="color:#212121">Pudavar</span></span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif;color:#212121"> </span></span><span style="font-family:"Arial",sans-serif;color:#212121">(Leica),<span class="apple-converted-space"> </span>Sergey
Suslov (TFS),<span class="apple-converted-space"> </span>and Zoltan<span class="apple-converted-space"> </span></span><span class="spelle"><span style="color:#212121">Metlagel</span></span><span style="font-family:"Arial",sans-serif;color:#212121">(TFS). Other
speakers to be confirmed!<span class="apple-converted-space"> </span></span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#212121"> </span><span style="color:#212121"><o:p></o:p></span></p>
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<b><span style="font-family:"Arial",sans-serif;color:black">Training topics will include:</span></b><span style="font-family:"Arial",sans-serif;color:black"> </span><span style="color:#212121"><o:p></o:p></span></p>
<ul style="margin-top:0in;caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px" type="disc">
<li class="MsoNormal" style="color:black;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters. </span><o:p></o:p></li><li class="MsoNormal" style="color:black;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Training in data acquisition workflows that include screening, data collection, and flexible scripting.</span><o:p></o:p></li><li class="MsoNormal" style="color:black;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging. </span><o:p></o:p></li><li class="MsoNormal" style="color:black;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2. </span><o:p></o:p></li><li class="MsoNormal" style="color:black;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Guidance in FIB-milling with<span class="apple-converted-space"> </span></span><span class="spelle">AutoTEM</span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif"> </span></span><span style="font-family:"Arial",sans-serif">and
advanced FIB workflows such as waffle milling and lift-out methods. </span><o:p></o:p></li></ul>
<p style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span style="font-family:"Arial",sans-serif;color:black;background:white">The morning theory sessions of the workshop will be hybrid and the afternoon practical sessions will be in-person only.</span><span style="font-family:"Arial",sans-serif;color:#212121"><o:p></o:p></span></p>
<p style="margin-bottom:6.0pt;caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<b><span style="font-family:"Arial",sans-serif;color:#212121">Registration:<span class="apple-converted-space"> </span></span></b><span style="font-family:"Arial",sans-serif;color:#212121">In-person space is limited, so we encourage you to apply at your earliest
convenience. For more details and registration, please visit our<span class="apple-converted-space"> </span><b><a href="https://urldefense.com/v3/__https://explore.wisc.edu/LP=2218__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73-kYXMM-A$" target="_blank" title="https://explore.wisc.edu/LP=2218"><span style="color:#0078D7">workshop page</span></a></b><span class="apple-converted-space"> </span>– <span class="apple-converted-space"> </span><b><i>Registration
closes August 1st, 2024</i></b><i>.</i><o:p></o:p></span></p>
<p style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<b><span style="font-family:"Arial",sans-serif;color:#212121">Questions: <span class="apple-converted-space"> </span></span></b><span style="font-family:"Arial",sans-serif;color:#212121">For inquiries regarding meeting content, registration, and local lodging,
please direct your questions to the following three contacts at<span class="apple-converted-space"> </span><a href="mailto:cotten@wisc.edu;ceevans3@wisc.edu;cryoem@biochem.wisc.edue" title="mailto:cotten@wisc.edu;ceevans3@wisc.edu;cryoem@biochem.wisc.edue"><span style="color:#0078D7">Email
Us</span></a>:<o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#212121">Alex Cotten (<a href="mailto:cotten@wisc.edu" title="mailto:cotten@wisc.edu"><span style="color:#0078D7">cotten@wisc.edu</span></a>)</span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#212121">Corina Elizabeth Evans (<a href="mailto:ceevans3@wisc.edu" title="mailto:ceevans3@wisc.edu"><span style="color:#0078D7">ceevans3@wisc.edu</span></a>)</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="caret-color: rgb(33, 33, 33);font-variant-caps: normal;orphans: auto;text-align:start;widows: auto;-webkit-text-stroke-width: 0px;word-spacing:0px">
<span style="font-family:"Arial",sans-serif;color:#212121">UW-Madison Cryo-EM (<a href="mailto:cryoem@biochem.wisc.edu" title="mailto:cryoem@biochem.wisc.edu"><span style="color:#0078D7">cryoem@biochem.wisc.edu</span></a>).</span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#002451"> </span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#212121">We look forward to welcoming you to this enriching event!</span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#002451"> </span><span style="color:#212121"><o:p></o:p></span></p>
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<span style="font-family:"Arial",sans-serif;color:#002451"> </span><span style="color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:2.0pt"><b><span style="font-family:"Arial",sans-serif;color:black">Corina Elizabeth Evans</span></b><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:2.0pt"><i><span style="font-family:"Arial",sans-serif;color:black">Administrative Assistant II</span></i><span style="color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:2.0pt"><span style="font-family:"Arial",sans-serif;color:#212121">D: (608) 262-7175</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:2.0pt"><span style="font-family:"Arial",sans-serif;color:black">E:<span class="apple-converted-space"> </span></span><span style="color:#212121"><a href="mailto:ceevans3@wisc.edu" title="mailto:ceevans3@wisc.edu"><span style="font-family:"Arial",sans-serif;color:#0078D7">ceevans3@wisc.edu</span></a><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Arial",sans-serif;color:black">E:<span class="apple-converted-space"><b> </b></span></span><span style="color:#212121"><a href="mailto:cryoem@biochem.wisc.edu" title="mailto:cryoem@biochem.wisc.edu"><span style="font-family:"Arial",sans-serif;color:#0078D7">cryoem@biochem.wisc.edu</span></a></span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif;color:#0086F0"> </span></span><b><span style="font-family:"Arial",sans-serif;color:#C5050C">|<span class="apple-converted-space"> </span></span></b><span style="color:#212121"><a href="https://urldefense.com/v3/__https://cryoem.wisc.edu/__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73_qh8QXHA$" title="https://cryoem.wisc.edu/"><span style="font-family:"Arial",sans-serif;color:#0078D7">https://cryoem.wisc.edu/</span></a><o:p></o:p></span></p>
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<a href="https://urldefense.com/v3/__https://cryoem.wisc.edu/__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73_qh8QXHA$" title="https://cryoem.wisc.edu/"><span style="font-family:"Arial",sans-serif;color:#002451;text-decoration:none"><img border="0" width="402" height="78" style="width:4.1875in;height:.8125in" id="Picture_x0020_2" src="cid:image001.jpg@01DABB4E.56865BA0" alt="A close-up of a white background
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