[3dem] small flashes in glow discharger?

Tobias Furstenhaupt furstenh at mpi-cbg.de
Tue Jul 16 05:57:09 PDT 2024


Dear colleagues,

we use the PELCO easiGlow to glow discharge our grids before plungefrezing (and for other uses) and pretty happy with it. But we noticed small flashes from the bottom (see attachment - sorry for the unstable focus). Cleaning with ethanol or isopropanol has no effect. Also the metal in this area looks less shiny and potentially has now a slightly rougher surface.
The quality of the glow discharge on our grids seems unchanged. Has anybody experiencied similar things and should we be worried?

cheers 
Tobias 

PS: if the attached video does not work for you, here another version:
https://urldefense.com/v3/__https://owncloud.mpi-cbg.de/index.php/s/ox67qdfPrREwrDn__;!!Mih3wA!BNvMHT2jzp_k9qHObg52qygdEWsHhp4-R065xzSAIV9B9qL0fXMnbJhrdBXxSoKtDvoCYoUwjjLBzARFAovGysA$ 

----------------------------------- 
Tobias Fürstenhaupt, PhD 
head of Electron Microscopy 
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) 
Pfotenhauerstrasse 108 
01307 Dresden, Germany 

mail: furstenh at mpi-cbg.de 
phone: (+49) (0)351 210-2690 
cell: (+49) (0)176 / 44498706 (NEW!)
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