[3dem] Postdoctoral position to work on the structure of bacteriophages and more generally on phage infection by cryo-EM/ET
schoehn
guy.schoehn at ibs.fr
Thu Oct 19 02:03:33 PDT 2023
Dear all
There is an open position at the IBS Grenoble, France
*Postdoc Position in Electron cryo-Microscopy of phage infection*
Job description and objectives
A post-doctoral position is available to study, by cryo-electron
microscopy, the mechanisms of host recognition and infection
triggerinthecaseof siphophage9N1, a bacteriophage that infects /Salmonella/.
Bacteriophages are bacterialvirusesthatrecognise very specifically their
host. Host recognition initiates perforation
oftheheavilyarmouredbacterial
cellwall,allowingtheinjectionofviralDNAintothehostcytoplasm, which leads
to viral replication, phage productionand eventually liberationof
thenewvirions.The project wishes to obtainthe structural details of host
recognition and infection trigger by solving the structure of the whole
virion before and after interaction with its receptor, /i.e./ a membrane
of lipopolysaccharide. This will be done by electron cryo-microscopy
(cryoEM) single particle analysis to provide atomic resolution of the
virion and electron cryo-tomography on mini-skinny cells to have a more
global view how the siphophages perforates the whole cell wall.
This project is a collaboration between two leading research teams in
the field of biochemistry, biophysics and structural biology of
bacteriophages and electron microscopy: the teams of Cécile Breyton
(IBS, Grenoble) and of Guy Schoehn (IBS, Grenoble) in an international
ANR consortium with Stefanie Barbirz (MSB, Berlin). IBS is hosting
state-of-the‐artplatforms in structural
biology,includinganelectronmicroscopyplatformincludinga Glacios equipped
with aK2directelectron detector. Access toKrioses is also available at
the neighbouring ESRF.Grenoble, capital of the French Alps, is a very
lively and international city. IBS is part of the Partnership for
Structural Biology, including the EMBL outstation, ILL and ESRF with ~
300 scientist interested in structural biology producing an extremely
dynamic and motivating scientific environment.
*The postdoctoral researcher will have to:*
- produce, purify and optimise phage, outer-membrane vesicle and
mini-skinny cell samples needed for the project,
- prepare the samples for the electron cryo-microscope (vitrification),
- collect images (conventional imaging and tomography) of the samples
using the latest generation of electron cryo-microscopes and/or interact
with the local, national international EM platforms,
- process the data by image analysis (Relion, CryoSparc, eman2…),
- interpret the 3D structures obtained including tracing the peptidic
chain into the EM Coulomb map,
- write scientific articles and present the data at conferences.
Qualifications
The successful candidate will hold a PhD and be an expert in single
particle and tomography cryo-electron microscopy (sample preparation,
data acquisition) as well as in advanced image analysis techniques
(Relion, CryoSparc, eman2…) and show strong interest for phage biology.
We seek a candidate motivated to work independently and who will
demonstrate healthy team work ethic. In addition, candidates are
required to demonstrate excellent written and verbal communication skills.
Job Details
*Financial support*: French National Research Agency (ANR, 2022). The
salary will be based on the CEA guidelines for post-doctoral positions.
The CEA provides generous vacation days, good benefits and health
insurance. Candidates with or without a post-doctoral experience are
encouraged to apply.
*Lo**cation*: Institute for Structural Biology (IBS), Grenoble France
https://urldefense.com/v3/__http://www.ibs.fr__;!!Mih3wA!DAHr634KP8pTre0yWzyMYPwJfN3rmqQsxCbkmF9msdc_aLf6ajqvjEasn3Yy5geDxZ5oq5SGjrPexVXLl1F4zow$
*Starting date*: January 2024
*Duration*:18 months to 2 years depending on experience
To Apply
Interested candidates should send their CV summarising qualifications
and experience, including the names and addresses of two referees and a
motivation letter to Cécile Breyton (orcid: 0000-0002-4382-0434,
<mailto:0000-0002-4382-0434,%20>cecile.breyton at ibs.fr) and Guy Schoehn
(orcid: 0000-0002-1459-3201, guy.schoehn at ibs.fr <mailto:guy.schoehn at ibs.fr>)
Sincerely yours
Guy and Cécile
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