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<p>Dear all</p>
<p>There is an open position at the IBS Grenoble, France</p>
<p>
</p>
<p style="tab-stops:6.0cm"><b><span style="font-size:18.0pt;
font-family:"TimesNewRomanPS",serif;mso-ansi-language:EN-GB"
lang="EN-GB">Postdoc Position
in Electron cryo-Microscopy of phage infection</span></b></p>
<h1><span style="mso-ansi-language:EN-GB" lang="EN-GB">Job
description and
objectives</span></h1>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;tab-stops:432.35pt"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">A post-doctoral position is available to
study, by cryo-electron
microscopy, </span><span
style="font-size:11.0pt;mso-fareast-font-family:
Cambria;mso-ansi-language:EN-GB" lang="EN-GB">the mechanisms of
host recognition and infection
trigger<span style="letter-spacing:1.65pt"> </span>in<span
style="letter-spacing:
1.75pt"> </span>the<span style="letter-spacing:1.65pt"> </span>case<span
style="letter-spacing:1.65pt"> </span>of siphophage<span
style="letter-spacing:
1.75pt"> </span>9N1, a bacteriophage that infects <i
style="mso-bidi-font-style:
normal">Salmonella</i></span><span style="font-size:11.0pt;
mso-ansi-language:EN-GB" lang="EN-GB">.</span><span
style="font-size:11.0pt;
mso-fareast-font-family:Cambria;mso-ansi-language:EN-GB"
lang="EN-GB"> </span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;tab-stops:432.35pt"><span
style="font-size:11.0pt;mso-fareast-font-family:
Cambria;mso-ansi-language:EN-GB" lang="EN-GB">Bacteriophages are
bacterial<span style="letter-spacing:.3pt"> </span>viruses<span
style="letter-spacing:.05pt">
that</span><span style="letter-spacing:.25pt"> recognise very
specifically
their host. Host recognition initiates perforation </span>of<span
style="letter-spacing:.2pt"> </span>the<span
style="letter-spacing:.45pt"> </span><span
style="letter-spacing:.05pt">h</span>eavily<span
style="letter-spacing:.45pt"> </span>armoured<span
style="letter-spacing:.2pt"> </span>bacterial cell<span
style="letter-spacing:
.35pt"> </span>wall,<span style="letter-spacing:.3pt"> </span>allowing<span
style="letter-spacing:.15pt"> </span>the<span
style="letter-spacing:.4pt"> </span>injection<span
style="letter-spacing:.2pt"> </span>of<span
style="letter-spacing:.45pt"> </span>viral<span
style="letter-spacing:.35pt"> </span>DNA<span
style="letter-spacing:.35pt"> </span>into<span
style="letter-spacing:.35pt"> </span>the<span
style="letter-spacing:.35pt"> </span>host<span
style="letter-spacing:.3pt"> </span>cytoplas<span
style="letter-spacing:.1pt">m</span>,
which leads to viral replication, phage production<span
style="letter-spacing:
.05pt"> </span>and eventually liberation<span
style="letter-spacing:.1pt"> of </span>the<span
style="letter-spacing:.35pt"> </span>new<span
style="letter-spacing:.3pt"> </span>virion<span
style="letter-spacing:.05pt">s</span>.<span
style="letter-spacing:.35pt"> </span>The
project wishes to <span style="mso-bidi-font-weight:bold">obtain<span
style="letter-spacing:-.05pt"> the structural details of
host recognition and
infection trigger by solving the structure of the whole
virion before and after
interaction with its receptor, <i>i.e.</i> a membrane of
lipopolysaccharide</span></span></span><span
style="font-size:11.0pt;mso-bidi-font-size:12.0pt;mso-fareast-font-family:Cambria;mso-bidi-font-family:Cambria;letter-spacing:-.05pt;mso-ansi-language:
EN-GB;mso-bidi-font-weight:bold" lang="EN-GB">. This will be
done by electron
cryo-microscopy (cryoEM) single particle analysis to provide
atomic resolution
of the virion and electron cryo-tomography on mini-skinny cells
to have a more
global view how the siphophages perforates the whole cell wall.
</span><span style="font-size:11.0pt;mso-ansi-language:EN-GB"
lang="EN-GB"></span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;tab-stops:432.35pt"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">This project is a collaboration </span><span
style="font-size:11.0pt;mso-bidi-font-size:12.0pt;mso-ansi-language:EN-GB"
lang="EN-GB">between
two leading research teams in the field of biochemistry,
biophysics and
structural biology of bacteriophages and electron microscopy:
the teams of
Cécile Breyton (IBS, Grenoble) and of Guy Schoehn (IBS,
Grenoble) in an international
ANR consortium with Stefanie Barbirz (MSB, Berlin). IBS is
hosting </span><span
style="font-size:11.0pt;mso-bidi-font-size:12.0pt;mso-fareast-font-family:Cambria;mso-bidi-font-family:Cambria;mso-ansi-language:EN-GB"
lang="EN-GB">stat<span style="letter-spacing:-.05pt">e</span>-of-the</span><span
style="font-size:11.0pt;mso-bidi-font-size:12.0pt;font-family:"Cambria",serif;
mso-fareast-font-family:Cambria;mso-bidi-font-family:Cambria;mso-ansi-language:
EN-GB" lang="EN-GB">‐</span><span
style="font-size:11.0pt;mso-bidi-font-size:
12.0pt;mso-fareast-font-family:Cambria;mso-bidi-font-family:Cambria;mso-ansi-language:
EN-GB" lang="EN-GB">art<span style="letter-spacing:1.35pt"> </span>platforms
in structural
biology,<span style="letter-spacing:-.35pt"> </span>including<span
style="letter-spacing:2.1pt"> </span>an<span
style="letter-spacing:2.35pt"> </span>electron<span
style="letter-spacing:2.1pt"> </span>microsco<span
style="letter-spacing:.05pt">p</span>y<span
style="letter-spacing:1.9pt"> </span>platform<span
style="letter-spacing:2.05pt">
</span>including<span style="letter-spacing:2.3pt"> </span>a
Glacios equipped
with a<span style="letter-spacing:2.4pt"> </span>K2<span
style="letter-spacing:
2.35pt"> </span>direct<span style="letter-spacing:-.2pt">
electron </span>detector</span><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB">.
Access to</span><span
style="font-size:11.0pt;mso-bidi-font-size:12.0pt;mso-fareast-font-family:Cambria;mso-bidi-font-family:Cambria;mso-ansi-language:EN-GB"
lang="EN-GB"> Krioses is also
available at the neighbouring ESRF.<span
style="letter-spacing:2.15pt"> </span></span><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB">Grenoble,
capital
of the French Alps, is a very lively and international city. IBS
is part of the
Partnership for Structural Biology, including the EMBL
outstation, ILL and ESRF
with ~ 300 scientist interested in structural biology producing
an extremely
dynamic and motivating scientific environment.</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;tab-stops:432.35pt"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB"> </span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;mso-pagination:none;tab-stops:432.35pt;mso-layout-grid-align:none;
text-autospace:none"><b><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">The postdoctoral researcher will have to:</span></b></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;mso-pagination:none;tab-stops:432.35pt;mso-layout-grid-align:none;
text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">- produce, purify and optimise phage,
outer-membrane vesicle and
mini-skinny cell samples needed for the project,</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;mso-pagination:none;tab-stops:432.35pt;mso-layout-grid-align:none;
text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">- prepare the samples for the electron
cryo-microscope (vitrification),</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;mso-pagination:none;tab-stops:432.35pt;mso-layout-grid-align:none;
text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">- collect images (conventional imaging and
tomography) of the samples
using the latest generation of electron cryo-microscopes and/or
interact with the
local, national international EM platforms,</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;mso-pagination:none;tab-stops:432.35pt;mso-layout-grid-align:none;
text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">- process the data by image analysis </span><span
style="font-size:11.0pt;mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;
mso-ansi-language:EN-GB" lang="EN-GB">(Relion, CryoSparc,
eman2…),</span><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB"></span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;mso-pagination:none;tab-stops:432.35pt;mso-layout-grid-align:none;
text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">- interpret the 3D structures obtained
including tracing the peptidic
chain into the EM Coulomb map,</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;mso-pagination:none;tab-stops:432.35pt;mso-layout-grid-align:none;
text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">- write scientific articles and present the
data at conferences.</span></p>
<h1><span style="mso-ansi-language:EN-GB" lang="EN-GB">Qualifications
</span><span
style="font-size:10.0pt;font-family:"SymbolMT",serif;mso-ansi-language:EN-GB"
lang="EN-GB"></span></h1>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;text-indent:
14.2pt;tab-stops:432.35pt"><span
style="font-size:11.0pt;mso-ansi-language:
EN-GB" lang="EN-GB">The successful candidate will hold a PhD and
be an expert in single
particle and tomography cryo-electron microscopy (sample
preparation, data </span><span
style="font-size:11.0pt;mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:EN-GB"
lang="EN-GB">acquisition) as well as in advanced image
analysis techniques (Relion, CryoSparc, eman2…) and show strong
interest for
phage biology. We seek a </span><span style="font-size:11.0pt;
mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:
EN-GB;mso-fareast-language:FR" lang="EN-GB">candidate motivated
to work independently and who
will demonstrate healthy team work ethic. In addition,
candidates are required
to demonstrate excellent written and verbal communication
skills.<span style="mso-spacerun:yes"> </span></span><span
style="font-size:
11.0pt;mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:
EN-GB" lang="EN-GB"></span></p>
<h1><span style="mso-ansi-language:EN-GB" lang="EN-GB">Job Details </span><span
style="font-size:10.0pt;font-family:"SymbolMT",serif;mso-ansi-language:
EN-GB" lang="EN-GB"></span></h1>
<p
style="margin-top:0cm;margin-right:0cm;margin-bottom:0cm;margin-left:17.85pt"><b
style="mso-bidi-font-weight:normal"><span
style="font-size:11.0pt;
font-family:"Calibri",sans-serif;mso-ascii-theme-font:minor-latin;mso-hansi-theme-font:
minor-latin;mso-bidi-theme-font:minor-latin;mso-ansi-language:EN-GB"
lang="EN-GB">Financial
support</span></b><span
style="font-size:11.0pt;font-family:"Calibri",sans-serif;
mso-ascii-theme-font:minor-latin;mso-hansi-theme-font:minor-latin;mso-bidi-theme-font:
minor-latin;mso-ansi-language:EN-GB" lang="EN-GB">: French
National Research Agency (ANR,
2022). The salary will be based on the CEA guidelines for
post-doctoral
positions. The CEA provides generous vacation days, good
benefits and health
insurance. Candidates with or without a post-doctoral experience
are encouraged
to apply.</span></p>
<p class="MsoNormal"
style="margin-top:0cm;margin-right:-.3pt;margin-bottom:0cm;
margin-left:18.0pt;margin-bottom:.0001pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><b><span
style="font-size:11.0pt;mso-bidi-font-family:Calibri;mso-bidi-theme-font:
minor-latin;mso-ansi-language:EN-GB" lang="EN-GB">Lo</span></b><b
style="mso-bidi-font-weight:
normal"><span
style="font-size:11.0pt;mso-bidi-font-family:Calibri;
mso-bidi-theme-font:minor-latin;mso-ansi-language:EN-GB"
lang="EN-GB">cation</span></b><span
style="font-size:11.0pt;mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:EN-GB"
lang="EN-GB">: Institute for Structural Biology (IBS),
Grenoble France <a class="moz-txt-link-abbreviated" href="https://urldefense.com/v3/__http://www.ibs.fr__;!!Mih3wA!DAHr634KP8pTre0yWzyMYPwJfN3rmqQsxCbkmF9msdc_aLf6ajqvjEasn3Yy5geDxZ5oq5SGjrPexVXLl1F4zow$">www.ibs.fr</a></span></p>
<p class="MsoNormal"
style="margin-top:0cm;margin-right:-.3pt;margin-bottom:0cm;
margin-left:18.0pt;margin-bottom:.0001pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><b
style="mso-bidi-font-weight:normal"><span
style="font-size:11.0pt;
mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:
EN-GB" lang="EN-GB">Starting date</span></b><span
style="font-size:11.0pt;
mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:
EN-GB" lang="EN-GB">: January 2024</span></p>
<p class="MsoNormal"
style="margin-top:0cm;margin-right:-.3pt;margin-bottom:0cm;
margin-left:18.0pt;margin-bottom:.0001pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><b
style="mso-bidi-font-weight:normal"><span
style="font-size:11.0pt;
mso-bidi-font-family:Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:
EN-GB" lang="EN-GB">Duration</span></b><span
style="font-size:11.0pt;mso-bidi-font-family:
Calibri;mso-bidi-theme-font:minor-latin;mso-ansi-language:EN-GB"
lang="EN-GB">:<span style="mso-spacerun:yes"> </span>18 months
to 2 years depending on experience</span></p>
<h1><span style="mso-ansi-language:EN-GB" lang="EN-GB">To Apply </span><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB"></span></h1>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB">Interested
candidates should send their CV summarising qualifications and
experience,
including the names and addresses of two referees and a
motivation letter to
Cécile Breyton (orcid: </span><a
href="mailto:0000-0002-4382-0434,%20"><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB">0000-0002-4382-0434,
</span></a><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB"><a class="moz-txt-link-abbreviated" href="mailto:cecile.breyton@ibs.fr">cecile.breyton@ibs.fr</a>)
and Guy Schoehn (orcid: 0000-0002-1459-3201, </span><a
href="mailto:guy.schoehn@ibs.fr"><span style="font-size:11.0pt;
mso-ansi-language:EN-GB" lang="EN-GB">guy.schoehn@ibs.fr</span></a><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB">)</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB"><br>
</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB"><br>
</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB">Sincerely
yours</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB"><br>
</span></p>
<p class="MsoNormal"
style="margin-right:-.3pt;text-align:justify;mso-pagination:
none;tab-stops:432.35pt;mso-layout-grid-align:none;text-autospace:none"><span
style="font-size:11.0pt;mso-ansi-language:EN-GB" lang="EN-GB">Guy
and Cécile<br>
</span></p>
<p>
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