[3dem] First Grenoble Practical Workshop on Helical Symmetry determination and reconstruction
Leandro F. Estrozi
leandro.estrozi at ibs.fr
Fri May 26 08:26:54 PDT 2023
Dear EM (helical assemblies interested) community,
We are really happy to announce the first Grenoble Practical Workshop
dedicated to helical symmetry determination of biological polymers
imaged by cryo-EM, *17th to 21st of July 2023, online*.
Determining the helical parameters from a sample with helical
symmetry(ies) is often the bottleneck for successful structure
determination. The learning curve to get familiar with this process is
steep and full of crevices.
In this workshop, we will guide the participants who already have
helical assemblies data in their hands (see below) through this process,
exploiting all possible information, including power spectra
interpretation, 2D class-averages analysis and clue hunting from raw
data inspection.
The practicals will cover the use of helixplorer
(https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!FuXAIKSush5n5zbFX1AKGJLHIyqhXwFf8VHpoYSSWGs8Pp3GEfVBZBiBYLlnaFEBrKTzfbA8VwHrMubwy_iaUharVpj2$ ), but will not be limited to this
approach.
Although the main focus will be on helical parameters determination, we
will also discuss general reconstruction strategies, including dealing
with flexible helices, multiple symmetries, and merging of data from
different symmetry filaments using subtraction approaches.
In order to apply to the workshop, applicants :
-can be at any career level (PhD, postdocs, senior scientists, ...)
-must have an ongoing project involving a helical sample, and have
difficulties in solving its structure
-agree to work on their own data during the workshop. Participants are
expected to share some raw images, 2D-class averages, and sum of PS with
the organizers (and screen sharing with other participants). However,
the name of their protein/organism and the exact nature and function of
their protein/filaments will remain confidential.
Please send your application, including a short letter describing your
project and difficulties, screenshots of raw data and 2D class-averages, to
ambroise.desfosses at ibs.fr
leandro.estrozi at ibs.fr
*Before the 15th of June. *
Looking forward,
Ambroise & Leandro
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