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<p>Dear EM (helical assemblies interested) community, <br>
<br>
We are really happy to announce the first Grenoble Practical
Workshop dedicated to helical symmetry determination of biological
polymers imaged by cryo-EM, <b>17th to 21st of July 2023, online</b>.
<br>
<br>
Determining the helical parameters from a sample with helical
symmetry(ies) is often the bottleneck for successful structure
determination. The learning curve to get familiar with this
process is steep and full of crevices. <br>
<br>
In this workshop, we will guide the participants who already have
helical assemblies data in their hands (see below) through this
process, exploiting all possible information, including power
spectra interpretation, 2D class-averages analysis and clue
hunting from raw data inspection. <br>
<br>
The practicals will cover the use of helixplorer (<a class="moz-txt-link-freetext" href="https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/helixplorer/__;!!Mih3wA!FuXAIKSush5n5zbFX1AKGJLHIyqhXwFf8VHpoYSSWGs8Pp3GEfVBZBiBYLlnaFEBrKTzfbA8VwHrMubwy_iaUrQhlR8j$">https://rico.ibs.fr/helixplorer/</a>),
but will not be limited to this approach. <br>
<br>
Although the main focus will be on helical parameters
determination, we will also discuss general reconstruction
strategies, including dealing with flexible helices, multiple
symmetries, and merging of data from different symmetry filaments
using subtraction approaches. <br>
<br>
<br>
In order to apply to the workshop, applicants : <br>
<br>
-can be at any career level (PhD, postdocs, senior scientists,
...) <br>
<br>
-must have an ongoing project involving a helical sample, and have
difficulties in solving its structure <br>
<br>
-agree to work on their own data during the workshop. Participants
are expected to share some raw images, 2D-class averages, and sum
of PS with the organizers (and screen sharing with other
participants). However, the name of their protein/organism and the
exact nature and function of their protein/filaments will remain
confidential. <br>
<br>
Please send your application, including a short letter describing
your project and difficulties, screenshots of raw data and 2D
class-averages, to <br>
<br>
<a class="moz-txt-link-abbreviated moz-txt-link-freetext"
href="mailto:ambroise.desfosses@ibs.fr">ambroise.desfosses@ibs.fr</a>
<br>
<br>
<a class="moz-txt-link-abbreviated moz-txt-link-freetext"
href="mailto:leandro.estrozi@ibs.fr">leandro.estrozi@ibs.fr</a>
<br>
<br>
<b>Before the 15th of June. </b><br>
<br>
Looking forward, <br>
<br>
Ambroise & Leandro </p>
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