[3dem] Quantifoil grids with continuous carbon
Cryo EM
cryoemfan35 at gmail.com
Mon Feb 13 05:32:17 PST 2023
Hi,
We have recently ordered a few grids from Quantifoil with R 3/3 on Cu 300
mesh grids + 3 nm C (Holey Carbon Films with ~ 3 nm continuous carbon on
top) configuration.
I have been observing recently that many of the grids of the batch have no
carbon in the hole when we screen them after sample freezing. Now, I am
wondering if it is possible/common that this problem can arise?
Otherwise, I would be more skeptical about our freezing or extended glow
discharge!
Thanks!
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20230213/89eed3da/attachment.html>
More information about the 3dem
mailing list