[3dem] icing with Gatan ELSA 698

[Michael Elbaum]

Hi Tim,


I agree with Marc, it sounds like residual water vapor that condenses on some other surface at -130C. Can you monitor the vacuum while doing a cryo-cycle, or at least do a very long one? We had a bug that set the default duration too short (on a Tecnai) and running the vacuum monitor was very useful. Or does the problem appear only with this particular holder?


Michael

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Marc Stuart <m.c.a.stuart at rug.nl>
Sent: Friday, October 21, 2022 9:29:02 AM
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] icing with Gatan ELSA 698

Hoi Tim,

It seems that your stage is doing fine but that there is a lot of
remaining water in the vacuum of your microscope which contaminates your
sample as soon as the temperature of the stage is below the temperature
at which water can sublimate. Check the vacuum and make sure that the
anti contaminator of the microscope is properly cooled.

Marc Stuart

University of Groningen, The Netherlands

Op 21/10/2022 om 00:25 schreef Tim Gruene:
> Hello erveryone,
>
> our Gatan ELSA 698 holder produces ice such that we see the ice rings
> in the ED diffraction pattern. The ice evaporates at -130C, but returns
> as soon as we reduce the temperature again below about -140C.
>
> This also occurs when we insert the holder at room temperature and only
> cool it once fully inserted into the TEM (JEOL JEM2100Plus)
>
> Would there be any ideas for the cause of this icing, and maybe even a
> cure?
>
> Thanks a lot!
> Best regards,
> Tim
>
>
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