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<p>Hi Tim,</p>
<p><br>
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<p>I agree with Marc, it sounds like residual water vapor that condenses on some other surface at -130C. Can you monitor the vacuum while doing a cryo-cycle, or at least do a very long one? We had a bug that set the default duration too short (on a Tecnai)
and running the vacuum monitor was very useful. Or does the problem appear only with this particular holder?</p>
<p><br>
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<p>Michael<br>
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<div id="x_divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Marc Stuart <m.c.a.stuart@rug.nl><br>
<b>Sent:</b> Friday, October 21, 2022 9:29:02 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] icing with Gatan ELSA 698</font>
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<div class="PlainText">Hoi Tim,<br>
<br>
It seems that your stage is doing fine but that there is a lot of <br>
remaining water in the vacuum of your microscope which contaminates your <br>
sample as soon as the temperature of the stage is below the temperature <br>
at which water can sublimate. Check the vacuum and make sure that the <br>
anti contaminator of the microscope is properly cooled.<br>
<br>
Marc Stuart<br>
<br>
University of Groningen, The Netherlands<br>
<br>
Op 21/10/2022 om 00:25 schreef Tim Gruene:<br>
> Hello erveryone,<br>
><br>
> our Gatan ELSA 698 holder produces ice such that we see the ice rings<br>
> in the ED diffraction pattern. The ice evaporates at -130C, but returns<br>
> as soon as we reduce the temperature again below about -140C.<br>
><br>
> This also occurs when we insert the holder at room temperature and only<br>
> cool it once fully inserted into the TEM (JEOL JEM2100Plus)<br>
><br>
> Would there be any ideas for the cause of this icing, and maybe even a<br>
> cure?<br>
><br>
> Thanks a lot!<br>
> Best regards,<br>
> Tim<br>
><br>
><br>
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