[3dem] [EXTERNAL] Uranyl acetate alternative

[Sharon G Wolf]

I have used sodium silicotungstate on a variety of samples.  Also phosphotungstic acid is a classic.
Also, like Zuben, I have used ammonium molybdate and methylamine tungstate.   Both need to be made fresh. They can't sit on the shelf for a long time like UAc can.
None of them has as much contrast as UAc, except for the sodium silicotungstate, which can be outstanding. But it can cause some complexes to fall apart.
Sharon



From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Brown, Zuben
Sent: Wednesday, 20 October, 2021 15:41
To: Denis Chrétien <denis.chretien at univ-rennes1.fr>; 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] [EXTERNAL] Uranyl acetate alternative

Hi Denis,

I've tried ammonium molybdate and methylamine tungstate with acceptable results on different membrane proteins.
Obviously, the contrast is reduced compared to uranyl acetate but the management of the waste materials is greatly simplified.

best wishes,
Zuben
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>> on behalf of Denis Chrétien <denis.chretien at univ-rennes1.fr<mailto:denis.chretien at univ-rennes1.fr>>
Sent: Wednesday, October 20, 2021 3:35 AM
To: 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu> <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>
Subject: [EXTERNAL] [3dem] Uranyl acetate alternative

Dear all,

It becomes difficult to posses Uranyl Acetate salts. Hence we would like to have your feedback concerning alternatives for sections and negative staining. Along this line, we would appreciate your experience concerning this salt :

https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwIFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=BYd6VJL1DhJbdine2tnizFq2Pc0-Yp-srH7z6u9Pqnc&s=3OkTNgEzYDW0sDKks5jygTqGnF10uU8oPYXxzJp9Fp8&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=SzdIxK_0wo-0i8bTfRNyPQnZX2l3AyLDYJwGNuZsMTs&e=>

Other suggestions are welcomed!

Best,

Denis
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Université de Rennes 1
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Campus de Beaulieu, Bât. 13
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France

Tel. +33 (0)2 23 23 67 64
https://urldefense.proofpoint.com/v2/url?u=https-3A__igdr.univ-2Drennes1.fr_&d=DwIFAw&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=BYd6VJL1DhJbdine2tnizFq2Pc0-Yp-srH7z6u9Pqnc&s=mZq6Xv4EHzpYYxfm88N6hmnUm3o7Xd6yoepNN77pN_I&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__igdr.univ-2Drennes1.fr_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=J9NRrJh9gl0QKmDtkcBNTdu6xKGp5rtgq6N8Gs-jWos&e=>
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