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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">I have used sodium silicotungstate on a variety of samples. Also phosphotungstic acid is a classic.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Also, like Zuben, I have used ammonium molybdate and methylamine tungstate. Both need to be made fresh. They can’t sit on the shelf for a long time like UAc
can.<br>
None of them has as much contrast as UAc, except for the sodium silicotungstate, which can be outstanding. But it can cause some complexes to fall apart.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Sharon<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Brown, Zuben<br>
<b>Sent:</b> Wednesday, 20 October, 2021 15:41<br>
<b>To:</b> Denis Chrétien <denis.chretien@univ-rennes1.fr>; 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] [EXTERNAL] Uranyl acetate alternative<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Hi Denis,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">I've tried ammonium molybdate and methylamine tungstate with acceptable results on different membrane proteins.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Obviously, the contrast is reduced compared to uranyl acetate but the management of the waste materials is greatly simplified.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">best wishes,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Zuben<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> 3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>>
on behalf of Denis Chrétien <<a href="mailto:denis.chretien@univ-rennes1.fr">denis.chretien@univ-rennes1.fr</a>><br>
<b>Sent:</b> Wednesday, October 20, 2021 3:35 AM<br>
<b>To:</b> <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a> <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>
<b>Subject:</b> [EXTERNAL] [3dem] Uranyl acetate alternative</span> <o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal">Dear all, <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">It becomes difficult to posses Uranyl Acetate salts. Hence we would like to have your feedback concerning alternatives for sections and negative staining. Along this line, we would appreciate your experience concerning this salt :<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=SzdIxK_0wo-0i8bTfRNyPQnZX2l3AyLDYJwGNuZsMTs&e=">https://www.tedpella.com/SDS_html/19485_sds.pdf</a><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Other suggestions are welcomed!<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Best,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Denis<o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="color:black">___________________________<br>
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<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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