[3dem] cryo TEM holder for autogrids and ThermoFisher/FEI

[Garry P Morgan]

Tobias,
we’ve been using the UH tilt Elsa holder over the last year…
pro’s:
-we get ~10-11 hours of stable imaging time for overnight acquisition
-it’s much more stable than our 626 holders

con’s:
-we see a higher occcurrence of crystalline/non-vitreous ice; i believe this is at least partly due to the thin tip on the UH tilt version of the holder
-more column vacuum crashes when inserting into our G2 series Tecnai TEMs; i’m pretty sure is due to the excessive weight of the holder and large dewar, especially when it’s on the full side with LN2.  i’ve actually come up with some tweaks to inserting the holder that have resulted in dramatically reducing the number of vacuum crashes…since this is a bit off-topic from the autogrid question, i won’t get into the details, but anyone interested in having fewer vacuum crashes please contact me.

i’m curious to know more about the Model 200 holder after your 2 week trial, as we’ll be looking into this as well in the very near future…i saw the response from Syzmon last week, but curious to know more:
-were you happy with the transfer station? is it comparable to the 626 station?
-is the process of loading auto-grids into this holder pretty straight forward and simple?
-how long does the LN2 last?
-how does the stability compare to a 626 holder?

cheers,
Garry

________________________________
Garry Morgan
Director  —  Boulder EM Services —  Department of MCD Biology
Campus Box 347  —  University of Colorado  —  Boulder, CO 80309
phone:  303-492-8402


On Jun 8, 2021, at 11:51 PM, Tobias Furstenhaupt <furstenh at mpi-cbg.de<mailto:furstenh at mpi-cbg.de>> wrote:

Dear colleagues,

So far we have two 626 Gatan cryoholders but we now look for a new cryo holder for our side-entry Cryo TEM (Titan Halo) to image autogrids. To my knowledge there are two companies offering those holders in the new 'toroid' design with temperature holding time of about 10h:

- Gatan with its "Elsa" holder for one or three autogrids
- Simple Origins Model 200 for two autogrids

does anybody of you know other sources? Do you have any experiences with the the Elsa or 200? We tested the latter for two weeks and it works as promised without problems.

cheers
Tobias


-----------------------------------
Tobias Fürstenhaupt, PhD
head of Electron Microscopy
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstrasse 108
01307 Dresden, Germany

mail: furstenh at mpi-cbg.de<mailto:furstenh at mpi-cbg.de>
phone: (+49) (0)351 210-2690
cell: (+49) (0)176 / 44498706 (NEW!)
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