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Tobias,
<div class="">we’ve been using the UH tilt Elsa holder over the last year…</div>
<div class="">pro’s: </div>
<div class="">-we get ~10-11 hours of stable imaging time for overnight acquisition</div>
<div class="">-it’s much more stable than our 626 holders</div>
<div class=""><br class="">
</div>
<div class="">con’s: </div>
<div class="">-we see a higher occcurrence of crystalline/non-vitreous ice; i believe this is at least partly due to the thin tip on the UH tilt version of the holder</div>
<div class="">-more column vacuum crashes when inserting into our G2 series Tecnai TEMs; i’m pretty sure is due to the excessive weight of the holder and large dewar, especially when it’s on the full side with LN2. i’ve actually come up with some tweaks to
inserting the holder that have resulted in dramatically reducing the number of vacuum crashes…since this is a bit off-topic from the autogrid question, i won’t get into the details, but anyone interested in having fewer vacuum crashes please contact me.</div>
<div class=""><br class="">
</div>
<div class="">i’m curious to know more about the Model 200 holder after your 2 week trial, as we’ll be looking into this as well in the very near future…i saw the response from Syzmon last week, but curious to know more:</div>
<div class="">-were you happy with the transfer station? is it comparable to the 626 station?</div>
<div class="">-is the process of loading auto-grids into this holder pretty straight forward and simple?<br class="">
<div class="">
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-how long does the LN2 last?</div>
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<div class="">-how does the stability compare to a 626 holder?</div>
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<div>cheers,</div>
<div>Garry</div>
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<div class="">________________________________</div>
<div class="">Garry Morgan</div>
<div class="">Director — Boulder EM Services — Department of MCD Biology<br class="">
<div style="font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal;" class="">
Campus Box 347 — University of Colorado — Boulder, CO 80309</div>
<div style="font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal;" class="">
phone: 303-492-8402</div>
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<blockquote type="cite" class="">
<div class="">On Jun 8, 2021, at 11:51 PM, Tobias Furstenhaupt <<a href="mailto:furstenh@mpi-cbg.de" class="">furstenh@mpi-cbg.de</a>> wrote:</div>
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<div class="">
<div class="">Dear colleagues,<br class="">
<br class="">
So far we have two 626 Gatan cryoholders but we now look for a new cryo holder for our side-entry Cryo TEM (Titan Halo) to image autogrids. To my knowledge there are two companies offering those holders in the new 'toroid' design with temperature holding time
of about 10h:<br class="">
<br class="">
- Gatan with its "Elsa" holder for one or three autogrids<br class="">
- Simple Origins Model 200 for two autogrids<br class="">
<br class="">
does anybody of you know other sources? Do you have any experiences with the the Elsa or 200? We tested the latter for two weeks and it works as promised without problems.<br class="">
<br class="">
cheers <br class="">
Tobias <br class="">
<br class="">
<br class="">
----------------------------------- <br class="">
Tobias Fürstenhaupt, PhD <br class="">
head of Electron Microscopy <br class="">
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) <br class="">
Pfotenhauerstrasse 108 <br class="">
01307 Dresden, Germany <br class="">
<br class="">
mail: <a href="mailto:furstenh@mpi-cbg.de" class="">furstenh@mpi-cbg.de</a> <br class="">
phone: (+49) (0)351 210-2690 <br class="">
cell: (+49) (0)176 / 44498706 (NEW!)<br class="">
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