[3dem] Cryo Storage

[Trepout Sylvain]

Dear John,

You won't find a solution against the ice forming on the polystyrene lid. I think the main majority of us have this issue.

But do you see ice contamination (small ice flakes) in the 50 ml Falcon or next to the grids in the sample puck ?

The bottom of my deewar has lots of ice flakes, but I do not see ice flakes in the sample puck, so I do not really care about it. I also use 50 ml Falcon tubes and I punch holes through the lid. I almost never get ice flakes in the Falcon tubes. The humidity is not controled in the room, so it varies, a lot.

Best wishes,
Sylvain
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Sylvain TREPOUT, PhD, HDR
IR2 Inserm
Plateforme de Microscopie Electronique
INSERM US43 / CNRS UMS2016
Institut Curie Centre De Recherche
Centre Universitaire, Bât. 101B-110-111-112
Rue Henri Becquerel
CS 90030
91401 ORSAY Cedex, FRANCE
Phone : +33 1 69 86 30 81
Fax : +33 1 69 07 53 27
________________________________
De : 3dem <3dem-bounces at ncmir.ucsd.edu> de la part de Watt, John Daniel <watt at lanl.gov>
Envoyé : jeudi 21 janvier 2021 17:19:01
À : 3dem at ncmir.ucsd.edu
Objet : [3dem] Cryo Storage


Dear Colleagues,


We are seeking good solutions for ice-free storage of vitrified samples. We use a Vitrobot and front loading TEMs only, so no autogrids. Currently we place the grids in a plastic sample puck, then into a 50 mL Falcon tube and store in a Worthington 35LDB LN2 dewar. We see some frosting of the polystyrene lid, but dry this periodically to minimize frost. Our lab is controlled to 40 % humidity, which unfortunately we cannot change.


Are there any good off-the-shelf solutions for ice-free storage that we are missing?


Many thanks,


John Watt, PhD

Center for Integrated Nanotechnologies

Los Alamos National Laboratory

Office: +1-505-284-0018

Cell: +1-505-206-1523

Email: watt at lanl.gov
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