[3dem] contamination from UltrAuFoil?
Mathew Peet
mpeet at mrc-lmb.cam.ac.uk
Mon Oct 12 07:56:38 PDT 2020
Dear Winnie,
These can be removed with 2% HCl. The method I've used is pick up each
grid with tweezer and then dunk in 2% HCl, 0.2% HCl, millipore, acetone
then IPA. Several seconds in each and enter and exit small container (~
25 ml) at different position. Dry by wicking tweezers and grid with
filter paper in laminar flow hood and place on filter paper.
For immediate use in water I avoided the drying with solvents and this
also worked.
Best regards,
Mathew Peet
On 12/10/2020 10:08, Winnie Ling wrote:
> Dear colleagues,
>
> The protein we work with behaves very well with UltrAuFoil but we are
> getting some particles of around 1-2nm all over the grids, some grids
> with more, some grids with less. We did not see the same particles
> with classic quantifoils (but the protein aggregates around the edges
> of the holes). Could the particles be Au debris from the UltrAuFoil?
>
> Have any of you had the same problem? Any solution?
>
> Thanks a lot,
>
> Winnie Ling
>
>
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