[3dem] [ext] Spots in micrographs
Schacherl, Magdalena
magdalena.schacherl at charite.de
Fri Mar 20 09:33:28 PDT 2020
Hi Saumya
Did you check the same grids with another protein (e.g. apoferritin) in another buffer (but same blotting paper, same water source for the humidifier, etc.)?
We used to have the same problem very occasionally with our old Vitrobot Mark II (see attached micrograph). We think (but do not have the final proof) that it could have been a problem with the humidifier.
Good luck with future grids.
Best regards,
Magdalena
Von: 3dem <3dem-bounces at ncmir.ucsd.edu> im Auftrag von "Bajaj Saumya (Dr)" <Saumya.Bajaj at ntu.edu.sg>
Datum: Freitag, 20. März 2020 um 17:15
An: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Betreff: [ext] [3dem] Spots in micrographs
Dear all,
We are trying to solve the structure of a membrane protein. We have recently started seeing black spots (~1nm) on our micrographs (image attached). The pattern is not uniform, as there are areas where there are no spots. We tried changing buffer and detergent stock – that did not help. The grids are commercial graphene oxide girds and our buffer contains 0.03% GDN detergent.
Have any of you noticed something similar, or can help throw some light on what it could be?
Thanks a lot.
Best regards,
Saumya
________________________________
CONFIDENTIALITY: This email is intended solely for the person(s) named and may be confidential and/or privileged. If you are not the intended recipient, please delete it, notify us and do not copy, use, or disclose its contents.
Towards a sustainable earth: Print only when necessary. Thank you.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20200320/2c0b7501/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: Bildschirmfoto 2019-04-05 um 22.03.18.png
Type: image/png
Size: 1350718 bytes
Desc: Bildschirmfoto 2019-04-05 um 22.03.18.png
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20200320/2c0b7501/attachment-0001.png>
More information about the 3dem
mailing list