[3dem] Spots in micrographs
Walsh, Richard Michael
Richard_Walsh at hms.harvard.edu
Fri Mar 20 09:19:11 PDT 2020
Hi Saumya,
If these spots are absent in thicker ice; the black spots you are seeing are likely denaturation at the air water interface because of thin ice.
best regards,
Richard M. Walsh , Ph.D.
The Harvard Cryo-EM
Center for Structural Biology
Harvard Medical School
250 Longwood Avenue
SGM 102
Boston MA 02115
Phone: (617) 432-9042
Email:Richard_Walsh at hms.harvard.edu<mailto:Richard_Walsh at hms.harvard.edu>
Web: cryoem.hms.harvard.edu<http://cryoem.hms.harvard.edu/>
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Bajaj Saumya (Dr) <Saumya.Bajaj at ntu.edu.sg>
Sent: Friday, March 20, 2020 5:49 AM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: [3dem] Spots in micrographs
Dear all,
We are trying to solve the structure of a membrane protein. We have recently started seeing black spots (~1nm) on our micrographs (image attached). The pattern is not uniform, as there are areas where there are no spots. We tried changing buffer and detergent stock – that did not help. The grids are commercial graphene oxide girds and our buffer contains 0.03% GDN detergent.
Have any of you noticed something similar, or can help throw some light on what it could be?
Thanks a lot.
Best regards,
Saumya
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