[3dem] Hexamer dissociate while freezing Cryo-EM grids
Farzad
farzaad at gmail.com
Thu Jun 4 13:34:07 PDT 2020
Dear All,
The other thing that should be considered is the low pH of UA staining
agent. This may affect the structure.
BR
Ferrie
On Thu, Jun 4, 2020, 22:28 Davide <dafloris at biophys.mpg.de> wrote:
> Hi Umar
>
> you can find very useful advice in our publication:
>
> *Protein denaturation at the air-water interface and how to prevent it*
>
> https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!WI2T8011J4hHlUPmqOlNq7gdeOBHNDC41J9XoHtC6PPIOAHHAjz0HU65M1ytR9W2YA$
> <https://urldefense.com/v3/__https://elifesciences.org/articles/42747__;!!Mih3wA!VfLdm8jk1oLaorfhmBpHU8SgDDDqoBFeugRuH6bNfz5IOsbAbVHiBNK7SqXAfdlwNw$>
>
> Best regards,
>
>
> Davide
> _________________________________________________
>
> Davide Floris
> PhD student
> Max Planck Institute of Biophysics
> Structural Biology Department
> Max-von-Laue Straße 3
> 60438 Frankfurt am Main
> Germany
>
> (L2.090): +49-69-6303-3037
> (B2.205): +49-69-6303-3049
>
> On 4. Jun 2020, at 21:24, Umar Farook <umarfarook12 at gmail.com> wrote:
>
> Dear All,
>
> I would like to ask the community for suggestions on how to overcome the
> issue of hexamer dissociating while freezing Cryo-EM grids, the negative
> staining seems to be perfect hexamer at 0.1 mg/ml concentration. But the
> sample seems to fall apart at 1.5 mg/ml in Cryo freezing, please advise,
> thank you.
>
> Best Regards,
> Umar
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