[3dem] LaB6 to cryo - upgrade suggestions
Andrei Moiseenko
postmoiseenko at gmail.com
Fri Jun 7 10:25:03 PDT 2019
Dear all,
I consider upgrading my microscope to a cryo-em. My purpose is to use it as
a screening device and also for low-resolution single particle data
acquisition. But I am not sure if our configuration can possibly fit this
purpose, so I'm seeking for an advise.
What we already have:
1. JEOL JEM-2100: 200kV, Cs 1.4, Сс 1.8, LaB6 gun
2. Anticontaminator: JEOL cryo-fin (not a twin-blade style).
3. Pretty fine Thon rings up to 5A at -1mkm defocus
4. Three condenser lenses, nice parallel illumination
5. Stable 1.0-1.1 eV electron energy spread, measured from EELS ZLP
6. Already set up single particle acquisition with serialEM for
negative stained samples.
What we consider to get:
1. Direct Electron DE-20 instead of a 2k CCD
2. Gatan cryo-transfer holder.
I would appreciate any opinion on this upgrade. Does it seem reasonable or
not?
I will also be very grateful for any suggestions about highest possible
resolution on such a system and about any important limitations that we
should be aware of.
Best regards,
Andrey Moiseenko
Electron Microscopy Lab @ Biology Department
Moscow State University
<mailto:postmoiseenko at gmail.com> postmoiseenko at gmail.com
skype: callmoiseenko
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