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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Dear all,<o:p></o:p></p><p class=MsoNormal>I consider upgrading my microscope to a cryo-em. My purpose is to use it as a screening device and also for low-resolution single particle data acquisition. But I am not sure if our configuration can possibly fit this purpose, so I’m seeking for an advise.<o:p></o:p></p><p class=MsoNormal>What we already have:<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>JEOL JEM-2100: 200kV, Cs 1.4, <span lang=RU>σΣ</span> 1.8, LaB6 gun<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Anticontaminator: JEOL cryo-fin (not a twin-blade style).<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>3.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Pretty fine Thon rings up to 5A at -1mkm defocus<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>4.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Three condenser lenses, nice parallel illumination<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>5.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Stable 1.0-1.1 eV electron energy spread, measured from EELS ZLP<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>6.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Already set up single particle acquisition with serialEM for negative stained samples.<o:p></o:p></p><p class=MsoNormal>What we consider to get:<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l1 level1 lfo2'><![if !supportLists]><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Direct Electron DE-20 instead of a 2k CCD<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l1 level1 lfo2'><![if !supportLists]><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Gatan cryo-transfer holder.<o:p></o:p></p><p class=MsoNormal>I would appreciate any opinion on this upgrade. Does it seem reasonable or not?<o:p></o:p></p><p class=MsoNormal>I will also be very grateful for any suggestions about highest possible resolution on such a system and about any important limitations that we should be aware of.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Best regards,<br>Andrey Moiseenko<o:p></o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Electron Microscopy Lab @ Biology Department<br>Moscow State University<br><a href="mailto:postmoiseenko@gmail.com"><span style='color:blue'>postmoiseenko@gmail.com</span></a> <br>skype: callmoiseenko<o:p></o:p></p></div></body></html>