[3dem] bad background in K2 images
Craig Yoshioka
yoshiokc at ohsu.edu
Mon Jan 28 18:02:30 PST 2019
Agreed, flip or 180º rotation.
On Jan 28, 2019, at 8:50 PM, Peter Shen <peter.shen at biochem.utah.edu<mailto:peter.shen at biochem.utah.edu>> wrote:
Hi Garry-- Looks like you just need to apply a vertical flip to your gain file.
-Peter
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From: 3dem [3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>] on behalf of Garry P Morgan [Garry.Morgan at Colorado.EDU<mailto:Garry.Morgan at Colorado.EDU>]
Sent: Monday, January 28, 2019 6:36 PM
To: 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
Subject: [3dem] bad background in K2 images
Hello,
we have been experiencing some strange patterns in our motion-corrected K2 data (see attached images)…these were collected using super-res mode and processed in cryo-sparc, so they are quite heavily filtered, which really accentuates the wavy pattern, which is not even noticeable when observing an aligned/summed images from SerialEM or GMS. has anyone seen this or something similar with their K2 acquired data? and are there any ideas on how to fix this?
thanks,
Garry
<image001.png><image002.png>
________________________________
Garry Morgan
Director — Boulder EM Services — Department of MCD Biology
Campus Box 347 — University of Colorado — Boulder, CO 80309
phone: 303-492-8402
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