[3dem] 3dem Digest, Vol 138, Issue 48

Wed Feb 20 01:08:05 PST 2019

Re:[3dem] Cryo FIB-SEMs, not Aquilos

Hi Melissa,

If you think the electron beam of Aquilos is not good enough for IB-SEM
volumetric reconstruction of resin embedded samples, maybe you could go to
some better SEM/FIB (helios for example) together with third-party transfer
assistance like Quorum transfer system?

Best,
Qiang

<3dem-request at ncmir.ucsd.edu> 于2019年2月20日周三 上午12:23写道：

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> Today's Topics:
>
>    1. FW: Postdoc Position in Cryo-EM at the University of Oxford
>       (peijun at strubi.ox.ac.uk)
>    2. Available PhD and postdoc/scientist positions at
>       Ernst-Ruska-Centre (Sachse, Carsten)
>    3. Cryo FIB-SEMs, not Aquilos (Chambers, Melissa G)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 19 Feb 2019 20:20:49 -0000
> From: <peijun at strubi.ox.ac.uk>
> To: <CCPEM at JISCMAIL.AC.UK>,     <3dem at ncmir.ucsd.edu>
> Subject: [3dem] FW: Postdoc Position in Cryo-EM at the University of
>         Oxford
> Message-ID: <011d01d4c890$9bae6350$d30b29f0$@strubi.ox.ac.uk>
> Content-Type: text/plain; charset="utf-8"
>
> I am posting the postdoc position below for a colleague in Oxford.
>
> Best,
>
> Peijun
>
> -------------------------
>
>
>
> Dear all
>
>
>
> We are looking for a Postdoctoral Research Scientist to join Professor
> Christian Siebold's group located in the Division of Structural Biology
> (STRUBI), University of Oxford.
>
>
>
> Research in the Siebold group is focused on the structural biology of
> mammalian cell surface signalling involved in embryonic development,
> neuronal guidance and stem cell regulation (see e.g. Byrne et al Nature
> 2016, Healey et al NSMB 2015, Bell et al Science 2013). The scope of the
> project involves cryo-EM analysis of membrane proteins and extracellular
> signalling assemblies. We are seeking a highly-motivated Postdoctoral
> Research Scientist to tackle this ambitious project. Experience in single
> particle cryo-EM of integral membrane proteins and/or large multi-protein
> signalling complexes is of advantage.
>
>
>
> For more details please see:
>
>
>
>
> https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.display_form?p_company=10
> <
> https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.display_form?p_company=10&p_internal_external=E&p_display_in_irish=N&p_process_type=&p_applicant_no=&p_form_profile_detail=&p_display_apply_ind=Y&p_refresh_search=Y&p_recruitment_id=139045>
> &p_internal_external=E&p_display_in_irish=N&p_process_type=&p_applicant_no=&p_form_profile_detail=&p_display_apply_ind=Y&p_refresh_search=Y&p_recruitment_id=139045
>
>
>
> Thanks,
>
> Christian
>
> ------------------------
> Christian Siebold, PhD
> Professor of Structural Biology
> Cancer Research UK Senior Research Fellow
>
> University of Oxford
> Wellcome Centre for Human Genetics
> Division of Structural Biology
> Roosevelt Drive, Oxford OX3 7BN
> United Kingdom
> Email: christian at strubi.ox.ac.uk <mailto:christian at strubi.ox.ac.uk>
> Phone: (+44)-1865-287564
> http://www.strubi.ox.ac.uk/research/christian-siebold
>
>
>
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> ------------------------------
>
> Message: 2
> Date: Tue, 19 Feb 2019 22:02:28 +0000
> From: "Sachse, Carsten" <c.sachse at fz-juelich.de>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Available PhD and postdoc/scientist positions at
>         Ernst-Ruska-Centre
> Message-ID: <EF291081-5042-4A63-9C6F-EBC0FE64D504 at fz-juelich.de>
> Content-Type: text/plain; charset="utf-8"
>
> Dear all,
>
> I would like to draw the attention to a series of cryo-EM related openings
> in our new Ernst-Ruska-Centre 3 for Structural Biology at the Research
> Centre J?lich (Germany):
> 1. Structural biology scientist for protein purification
> 2. Cryo-EM specialist
> 3. PhD positions on autophagy and cryo-EM method development
>
> The Ernst Ruska-Centre (ER-C) at the Forschungszentrum J?lich houses some
> of the world's most advanced electron microscopes and tools for
> nanocharacterisation. The scientific research covers current issues in
> condensed matter physics and from now on cryo-EM on biomacromolecules and
> in the future development of electron microscopy. The ER-C has a total of
> 13 electron microscopes including the PICO FEI Titan with a point
> resolution of 0.5 ?. The facility has been extended with state-of-the-art
> cryo-microscopes FEI Talos Arctica including Bioquantum K3 and FEI Talos
> 120. The Structural Biology division of the ER-C investigates the
> structural and molecular mechanism of autophagy and pushes the development
> of cryo-EM related methodology. The J?lich campus hosts a vibrant electron
> microscopy, biophysics and structural biology community.
>
> Positions in summary:
>
> http://www.fz-juelich.de/er-c/er-c-3/EN/Karriere/_Stellenangebote/alleStellenangebote_node.html
> Closing date is March 5th, 2019.
>
>
> Feel free to contact me on any questions and to forward the information to
> the appropriate candidates.
>
> Best wishes,
>
>
> Carsten
> _____________________________________________________________
> Prof. Dr. Carsten Sachse
> Forschungszentrum J?lich
> Ernst Ruska- Centre for Microscopy and Spectroscopy with Electrons / ER-C3
> Structural Biology
> Wilhem-Johnen-Stra?e, 52425 J?lich
> Germany
> http://www.fz-juelich.de/er-c/er-c-3/
>
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> ------------------------------
>
> Message: 3
> Date: Tue, 19 Feb 2019 23:19:25 +0000
> From: "Chambers, Melissa G" <melissa.g.chambers at Vanderbilt.Edu>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Cryo FIB-SEMs, not Aquilos
> Message-ID: <F3C06FE8-7D86-4A54-B18B-27971B9F1EDD at vanderbilt.edu>
> Content-Type: text/plain; charset="utf-8"
>
> Does anyone have experience with cryo FIB-SEMs that are not the Aquilos?
> Perhaps Zeiss Crossbeam or TESCAN systems?
> We are interested in a range of uses from preparing lamella for cryo-ET in
> our Krios to FIB-SEM volumetric reconstruction of resin embedded samples.
>
> Any feedback is appreciated!
>
> Thanks,
> Melissa
>
>
> -----
> Melissa G. Chambers
>
> Director, Vanderbilt Cryo-EM Facility
> Center for Structural Biology
>
> Vanderbilt University
> RR1205 MCN
> 1161 21st Ave S
> Nashville TN 37232
>
> 615-322-4671
>
>
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> End of 3dem Digest, Vol 138, Issue 48
> *************************************
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-- 
Qiang (John) Guo
Department of Molecular Structural Biology
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
Email: guoq.tiger at gmail.com
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