<div dir="ltr"><div>Re:[3dem] Cryo FIB-SEMs, not Aquilos</div><div><br></div><div>Hi Melissa,</div><div><br>
</div><div>If you think the electron beam of Aquilos is not good enough for IB-SEM volumetric reconstruction of resin embedded samples, maybe you could go to some better SEM/FIB (helios for example) together with third-party transfer assistance like Quorum transfer system? <br></div><div><br></div><div>Best,</div><div>Qiang<br></div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr"><<a href="mailto:3dem-request@ncmir.ucsd.edu">3dem-request@ncmir.ucsd.edu</a>> 于2019年2月20日周三 上午12:23写道：<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Send 3dem mailing list submissions to<br>
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Today's Topics:<br>
<br>
1. FW: Postdoc Position in Cryo-EM at the University of Oxford<br>
(<a href="mailto:peijun@strubi.ox.ac.uk" target="_blank">peijun@strubi.ox.ac.uk</a>)<br>
2. Available PhD and postdoc/scientist positions at<br>
Ernst-Ruska-Centre (Sachse, Carsten)<br>
3. Cryo FIB-SEMs, not Aquilos (Chambers, Melissa G)<br>
<br>
<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Tue, 19 Feb 2019 20:20:49 -0000<br>
From: <<a href="mailto:peijun@strubi.ox.ac.uk" target="_blank">peijun@strubi.ox.ac.uk</a>><br>
To: <<a href="mailto:CCPEM@JISCMAIL.AC.UK" target="_blank">CCPEM@JISCMAIL.AC.UK</a>>, <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] FW: Postdoc Position in Cryo-EM at the University of<br>
Oxford<br>
Message-ID: <011d01d4c890$9bae6350$d30b29f0$@<a href="http://strubi.ox.ac.uk" rel="noreferrer" target="_blank">strubi.ox.ac.uk</a>><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
I am posting the postdoc position below for a colleague in Oxford.<br>
<br>
Best,<br>
<br>
Peijun<br>
<br>
-------------------------<br>
<br>
<br>
<br>
Dear all<br>
<br>
<br>
<br>
We are looking for a Postdoctoral Research Scientist to join Professor Christian Siebold's group located in the Division of Structural Biology (STRUBI), University of Oxford.<br>
<br>
<br>
<br>
Research in the Siebold group is focused on the structural biology of mammalian cell surface signalling involved in embryonic development, neuronal guidance and stem cell regulation (see e.g. Byrne et al Nature 2016, Healey et al NSMB 2015, Bell et al Science 2013). The scope of the project involves cryo-EM analysis of membrane proteins and extracellular signalling assemblies. We are seeking a highly-motivated Postdoctoral Research Scientist to tackle this ambitious project. Experience in single particle cryo-EM of integral membrane proteins and/or large multi-protein signalling complexes is of advantage.<br>
<br>
<br>
<br>
For more details please see:<br>
<br>
<br>
<br>
<a href="https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.display_form?p_company=10" rel="noreferrer" target="_blank">https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.display_form?p_company=10</a> <<a href="https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.display_form?p_company=10&p_internal_external=E&p_display_in_irish=N&p_process_type=&p_applicant_no=&p_form_profile_detail=&p_display_apply_ind=Y&p_refresh_search=Y&p_recruitment_id=139045" rel="noreferrer" target="_blank">https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.display_form?p_company=10&p_internal_external=E&p_display_in_irish=N&p_process_type=&p_applicant_no=&p_form_profile_detail=&p_display_apply_ind=Y&p_refresh_search=Y&p_recruitment_id=139045</a>> &p_internal_external=E&p_display_in_irish=N&p_process_type=&p_applicant_no=&p_form_profile_detail=&p_display_apply_ind=Y&p_refresh_search=Y&p_recruitment_id=139045<br>
<br>
<br>
<br>
Thanks,<br>
<br>
Christian<br>
<br>
------------------------<br>
Christian Siebold, PhD<br>
Professor of Structural Biology<br>
Cancer Research UK Senior Research Fellow<br>
<br>
University of Oxford<br>
Wellcome Centre for Human Genetics<br>
Division of Structural Biology<br>
Roosevelt Drive, Oxford OX3 7BN<br>
United Kingdom<br>
Email: <a href="mailto:christian@strubi.ox.ac.uk" target="_blank">christian@strubi.ox.ac.uk</a> <mailto:<a href="mailto:christian@strubi.ox.ac.uk" target="_blank">christian@strubi.ox.ac.uk</a>> <br>
Phone: (+44)-1865-287564<br>
<a href="http://www.strubi.ox.ac.uk/research/christian-siebold" rel="noreferrer" target="_blank">http://www.strubi.ox.ac.uk/research/christian-siebold</a><br>
<br>
<br>
<br>
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Message: 2<br>
Date: Tue, 19 Feb 2019 22:02:28 +0000<br>
From: "Sachse, Carsten" <<a href="mailto:c.sachse@fz-juelich.de" target="_blank">c.sachse@fz-juelich.de</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] Available PhD and postdoc/scientist positions at<br>
Ernst-Ruska-Centre<br>
Message-ID: <<a href="mailto:EF291081-5042-4A63-9C6F-EBC0FE64D504@fz-juelich.de" target="_blank">EF291081-5042-4A63-9C6F-EBC0FE64D504@fz-juelich.de</a>><br>
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<br>
Dear all,<br>
<br>
I would like to draw the attention to a series of cryo-EM related openings in our new Ernst-Ruska-Centre 3 for Structural Biology at the Research Centre J?lich (Germany):<br>
1. Structural biology scientist for protein purification<br>
2. Cryo-EM specialist<br>
3. PhD positions on autophagy and cryo-EM method development<br>
<br>
The Ernst Ruska-Centre (ER-C) at the Forschungszentrum J?lich houses some of the world's most advanced electron microscopes and tools for nanocharacterisation. The scientific research covers current issues in condensed matter physics and from now on cryo-EM on biomacromolecules and in the future development of electron microscopy. The ER-C has a total of 13 electron microscopes including the PICO FEI Titan with a point resolution of 0.5 ?. The facility has been extended with state-of-the-art cryo-microscopes FEI Talos Arctica including Bioquantum K3 and FEI Talos 120. The Structural Biology division of the ER-C investigates the structural and molecular mechanism of autophagy and pushes the development of cryo-EM related methodology. The J?lich campus hosts a vibrant electron microscopy, biophysics and structural biology community.<br>
<br>
Positions in summary:<br>
<a href="http://www.fz-juelich.de/er-c/er-c-3/EN/Karriere/_Stellenangebote/alleStellenangebote_node.html" rel="noreferrer" target="_blank">http://www.fz-juelich.de/er-c/er-c-3/EN/Karriere/_Stellenangebote/alleStellenangebote_node.html</a> <br>
Closing date is March 5th, 2019. <br>
<br>
<br>
Feel free to contact me on any questions and to forward the information to the appropriate candidates.<br>
<br>
Best wishes,<br>
<br>
<br>
Carsten<br>
_____________________________________________________________<br>
Prof. Dr. Carsten Sachse <br>
Forschungszentrum J?lich<br>
Ernst Ruska- Centre for Microscopy and Spectroscopy with Electrons / ER-C3 Structural Biology<br>
Wilhem-Johnen-Stra?e, 52425 J?lich<br>
Germany<br>
<a href="http://www.fz-juelich.de/er-c/er-c-3/" rel="noreferrer" target="_blank">http://www.fz-juelich.de/er-c/er-c-3/</a><br>
<br>
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Message: 3<br>
Date: Tue, 19 Feb 2019 23:19:25 +0000<br>
From: "Chambers, Melissa G" <melissa.g.chambers@Vanderbilt.Edu><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] Cryo FIB-SEMs, not Aquilos<br>
Message-ID: <<a href="mailto:F3C06FE8-7D86-4A54-B18B-27971B9F1EDD@vanderbilt.edu" target="_blank">F3C06FE8-7D86-4A54-B18B-27971B9F1EDD@vanderbilt.edu</a>><br>
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<br>
Does anyone have experience with cryo FIB-SEMs that are not the Aquilos? Perhaps Zeiss Crossbeam or TESCAN systems?<br>
We are interested in a range of uses from preparing lamella for cryo-ET in our Krios to FIB-SEM volumetric reconstruction of resin embedded samples.<br>
<br>
Any feedback is appreciated!<br>
<br>
Thanks,<br>
Melissa<br>
<br>
<br>
-----<br>
Melissa G. Chambers<br>
<br>
Director, Vanderbilt Cryo-EM Facility<br>
Center for Structural Biology<br>
<br>
Vanderbilt University<br>
RR1205 MCN<br>
1161 21st Ave S<br>
Nashville TN 37232<br>
<br>
615-322-4671<br>
<br>
<br>
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End of 3dem Digest, Vol 138, Issue 48<br>
*************************************<br>
</blockquote></div><br clear="all"><br>-- <br><div dir="ltr" class="gmail_signature"><div dir="ltr">Qiang (John) Guo<br>Department of Molecular Structural Biology<br>Max Planck Institute of Biochemistry<br>Am Klopferspitz 18<br>82152 Martinsried<br>Germany<br>Email: <a href="mailto:guoq.tiger@gmail.com" target="_blank">guoq.tiger@gmail.com</a> <br></div></div>