[3dem] GroEL best resolution map. (FSC RESOLUTION ??)
Marin van Heel
marin.vanheel at googlemail.com
Sat Sep 1 10:43:09 PDT 2018
Dear Natesh,
I just checked the technical data of the EMD-3407 dataset and I saw that
its pixel size is 1.05A . That means that no resolution levels better
than 3 x 1.05A = 3.15A may be claimed for processing results based on
that data set, following the standards we set in the past (for the
younger generation: I am the main inventor of the FSC ;) ). Going beyond
a resolution claim of 3.15A would mean that the dataset is undersampled,
and would thus require a new data collection with a smaller pixel size.
For a possible resolution claim of, say, 2A you need a maximum pixel
size of 0.66A ! Moreover, the 0.143 threshold FSC threshold used in
this EMD-3407 case is itself wrong, while based on sloppy statistics.
(https://www.biorxiv.org/content/early/2017/11/24/224402).Bottom line:
to compare the results of different cryo-EM experiments you need correct
resolution criteria applied to sufficiently fine sampled cryo-EM data.
Cheers
Marin
On 01/09/2018 12:57, Natesh Ramanathan wrote:
> Dear All,
>
> Does anyone know/have a GroEL cryoEM map with resolution 2.5 Å
> or better?
> I see that the best resolution in EMDataBank is 3.26 Å - EMD-3407 and
> EMD-3415.
> If you have one, is it possible to share the map with me?
>
> Many thanks.
> Best regards,
> Natesh
> --
> ----------------------------------------------------------
> "Live Simply and do Serious Things .. "
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> "In Science truth always wins"
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> ----------------------------------------------------------
> Dr. Ramanathan Natesh
> Assistant Professor,
> School of Biology,
> Indian Institute of Science Education and Research Thiruvananthapuram
> (IISER-TVM),
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