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Dear Natesh,<br>
<br>
I just checked the technical data of the EMD-3407 dataset and I
saw that its pixel size is 1.05A . That means that no resolution
levels better than 3 x 1.05A = 3.15A may be claimed for
processing results based on that data set, following the standards
we set in the past (for the younger generation: I am the main
inventor of the FSC ;) ). Going beyond a resolution claim of 3.15A
would mean that the dataset is undersampled, and would thus
require a new data collection with a smaller pixel size. For a
possible resolution claim of, say, 2A you need a maximum pixel
size of 0.66A ! Moreover, the 0.143 threshold FSC threshold used
in this EMD-3407 case is itself wrong, while based on sloppy
statistics.
(<a class="moz-txt-link-freetext" href="https://www.biorxiv.org/content/early/2017/11/24/224402">https://www.biorxiv.org/content/early/2017/11/24/224402</a>).Bottom
line: to compare the results of different cryo-EM experiments you
need correct resolution criteria applied to sufficiently fine
sampled cryo-EM data.<br>
<br>
Cheers<br>
<br>
Marin<br>
<br>
On 01/09/2018 12:57, Natesh Ramanathan wrote:<br>
</div>
<blockquote type="cite"
cite="mid:CAFT3tKskDTkbXBT8Qc3rJV=te7LjhRZEZjBfFwfWHLSV=65Bhw@mail.gmail.com">
<meta http-equiv="content-type" content="text/html; charset=utf-8">
<div dir="ltr">Dear All,
<div><br>
<div> Does anyone know/have a GroEL cryoEM map with
resolution 2.5 <span
style="color:rgb(0,0,0);font-family:verdana,DejaVu,sans-serif;font-size:12px;text-align:justify">Å</span>
or better?</div>
<div>
<div>I see that the best resolution in EMDataBank is <span
style="color:rgb(0,0,0);font-family:verdana,DejaVu,sans-serif;font-size:12px;text-align:justify">3.26
Å</span> - EMD-3407 and EMD-3415.<br>
</div>
<div> </div>
<div> If you have one, is it possible to share the map
with me? </div>
<div><br>
</div>
<div>Many thanks.</div>
<div>Best regards,<br>
Natesh</div>
-- <br>
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<div dir="ltr">----------------------------------------------------------<br>
"Live Simply and do Serious Things .. "<br>
- Dorothy Mary Crowfoot Hodgkin OM, FRS<br>
<br>
"In Science truth always wins"<br>
- Max Ferdinand Perutz OM FRS<br>
----------------------------------------------------------<br>
Dr. Ramanathan Natesh<br>
Assistant Professor, </div>
<div>School of Biology,</div>
<div dir="ltr">Indian Institute of Science
Education and Research Thiruvananthapuram
(IISER-TVM),<br>
<div>
Maruthamala P.O., Vithura,<br>
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Thiruvananthapuram, 695551, Kerala, India<br>
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target="_blank" moz-do-not-send="true">natesh@iisertvm.ac.in</a><br>
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target="_blank" moz-do-not-send="true">http://faculty.iisertvm.ac.in/natesh</a></div>
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<pre class="moz-signature" cols="72">--
==============================================================
Prof Dr Ir Marin van Heel
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
tel: +55-19-3518-2316
mobile +55-19-983455450 (current)
mobile +55-19-981809332
(041-19-981809332 TIM)
Skype: Marin.van.Heel
email: marin.vanheel(A_T)gmail.com
marin.vanheel(A_T)lnnano.cnpem.br
and: mvh.office(A_T)gmail.com
--------------------------------------------------
Emeritus Professor of Cryo-EM Data Processing
Leiden University
Mobile NL: +31(0)652736618 (ALWAYS ACTIVE SMS)
--------------------------------------------------
Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
email: m.vanheel(A_T)imperial.ac.uk
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I receive many emails per day and, although I try,
there is no guarantee that I will actually read each incoming email. </pre>
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