[3dem] Large image box size in CryoSparc2 and Relion3
Elena Orlova
e.orlova at mail.cryst.bbk.ac.uk
Mon Oct 22 09:42:29 PDT 2018
Hi Everybody,
I wonder if you can help me, any suggestions are welcome:
We need to process particles that are big enough and fit only in the
boxes of the size 900x900. I do not want to coarsen them.
Is there any way I can process them in CryoSparc (ver 2), or Relion 3?
So far CryoSparc2 crashes at 2D classification complaining that :
*cufftAllosFailed*
If I want to have 100 or 20 classes it fails in ~5min, with 5 classes
it failed after iteration #19. The error message is the same.
Relion 3 was more efficient: running two (2) month for 50 classes
reaching the iteration #2. Then the guy was mercilessly killed.
I am using 4 GPU with the memory of ~ 12Gb each
Set consists of ~ 35 000 images.
Many thanks in advance. Elena
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Elena Orlova
Crystallography, Institute for Structural and Molecular Biology,
Department of Biological Sciences
Birkbeck, University of London,
Malet Street,
LONDON WC1E 7HX
Tel: (+44) (0)20 7631 6845
Fax: (+44) (0)20 7631 6803
e-mail: e.orlova at mail.cryst.bbk.ac.uk
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