[3dem] [ccpem] lost gain reference image (Camera Normalization)
Marin van Heel
marin.vanheel at googlemail.com
Tue Oct 2 08:51:07 PDT 2018
Dear Dimitry,
We have shown that you can perform movie alignments on a more local
basis without using very extreme low-pass filters (sometimes described
as "B-factors"). Thus you will necessarily have a larger yield of usable
particles from the same set of micrographs. That is more than sufficient
evidence of improvement! The FRC is a metric that is local to your
correction operation and that measures the improvement directly. The
final 3D map resolution only comes at the end of a long pipeline, that
any two people will perform differently and that is too indirectly
related to the very early data-set correction. Bottom line: the FRC
metric is necessary and sufficient to show the data-set improvement by
the camera correction. However it does not necessarily and sufficiently
guarantee that nobody will generate gold-standard garbage further down
the pipeline. ;)
Cheers,
Marin
On 02/10/2018 07:54, Dimitry Tegunov wrote:
> Dear Marin,
>
> do you have results showing that the proposed correction improves the
> final map resolution vs. conventionally gain-corrected movies? I think
> the FRC curves are necessary and sufficient proof , but not sufficient
> to prove the advantage of your approach.
>
> Cheers,
> Dimitry
>
> On Mon, Oct 1, 2018 at 9:23 PM Marin van Heel
> <0000057a89ab08a1-dmarc-request at jiscmail.ac.uk
> <mailto:0000057a89ab08a1-dmarc-request at jiscmail.ac.uk>> wrote:
>
> Dear Da,
>
> In IMAGIC-4D you can perform the necessary camera correction!
> (https://www.nature.com/articles/srep10317). It does it better
> than any
> manufactures correction and improves the data significantly even when
> performed after using the standard gain correction.
>
> Cheers,
>
> Marin
>
>
> =====================================================
>
> On 01/10/2018 15:36, Da Cui wrote:
> > Hi all,
> > The gain reference image for one dataset was missing by
> accident. In order to achieve a more accurate motioncor result,
> does anyone have idea about how to generate a gain reference image
> from the dataset (around 3k movies)?
> > Thank you so much for your help!!!
> > ---Da
> >
> >
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