[3dem] Ice thickness
Sacha De Carlo
sachadecarlo at yahoo.com
Mon Feb 26 08:09:18 PST 2018
Unfortunately the molecular weight of the complex alone is useless to determine “proper” settings for the vitrobot. Buffer composition, sample concentration, etc would be required to give you an educated guess ...
CheersSacha
Sent from Yahoo Mail for iPhone
On Monday, February 26, 2018, 5:05 PM, Sanchaita Das <sanchaita06 at gmail.com> wrote:
Dear all,Does anyone have suggestions about what vitrobot settings may work for getting good single particles for a protein that is aproox 300KDa?I know its trial and error but if something might work for smaller proteins would love suggestions.Best,Sanchaita
I think 99 times and I find nothing.
I stop thinking,
swim in silence,
and the truth comes to me.
- Albert Einstein -
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