[3dem] Important RELION update
Bjoern Forsberg
bjorn.forsberg at dbb.su.se
Thu Aug 17 10:19:07 PDT 2017
Dear RELION-users,
With RELION version 2.0.4, a bug was introduced wherein each iteration
of auto-refinement incorrectly used the same (identical) half-set
reconstruction for alignment of all data. A degree of reference-bias is
therefore present, with the effect that the reported resolution is
higher than should be under "gold-standard" conditions. The FSC and
reported resolution is still based on comparing two half-set
reconstructions built from completely separate image data, but
reconstructions are not entirely free from over-fitting artifacts.
*This bug only affects refinements using half-sets, all classification
results are unaffected and can be considered entirely correct. *
In order to claim a so-called "gold-standard" resolution, versions
before and including 2.0.3, or version 2.1b1 (which is now available at
https://github.com/3dem/relion) should be used. *This is strongly
recommended*. There are also patches attached in case you require a
specific version to run refinement. To apply a patch, simply go to your
downloaded relion (github) directory and run
git apply 2.<version>_AR.patch
and then build and install.
We apologize for any inconvenience, but more importantly thank the
community for making us aware of any issues. In this particular case
Prof. Leonid Sazanov and Dr. Radostin Danev are both to credit, making
sure RELION continues to produce reliable results.
Sincerely,
Björn Forsberg
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