[3dem] THE SEVENTH BRAZIL SCHOOL FOR SINGLE PARTICLE CRYO-EM (SECOND ANNOUNCEMENT)
Marin van Heel
marin.vanheel at googlemail.com
Fri Dec 18 07:30:47 PST 2015
NOTE: We have experienced a very strong interest in the 7th Brazil
school since its first announcement. The school is already likely to be
oversubscribed so don't delay your registration!
_Second announcement:_
_THE SEVENTH BRAZIL SCHOOL FOR SINGLE PARTICLE CRYO-EM._
11-23 August 2016 (São Paulo, Brazil) (www.single-particles.org/school).
Our single-particle school is a unique intensive 12-day hands-on
course/workshop, in which theory and practice are tightly intertwined.
The understanding of the principles of cryo-EM data processing stands
central in this event and a few pioneers will explain the approaches to
the participants to the level where they can apply it themselves. The
spectrum of topics covered suits attendees who are new to the field as
well as those for whom the cryo-EM technique is of growing importance
and who wish to acquire an in-depth understanding of its fundamental
principles.
The final days of the meeting will be devoted to advanced topics such as
high-resolution refinements and the processing of heterogeneous data
sets ("4D cryo–EM”). Advanced topics will include:
1)Optimizing data collection with direct electrons detectors and
exploiting movies.
2)Full data set automatic CTF correction.
3)Correction of anisotropic magnification.
4)Reaching atomic resolution without reference bias:
alignment-by-classification (ABC) refinement procedures.
5)Unbiased "Random Startup" of 3D and 4D reconstructions.
6)4D refinement procedures for high resolution results using IMAGIC-4D
and/or Frealign.
7)Fitting and refining atomic coordinates in high-resolution cryo-EM
maps (~4Å or better) using programs like REFMAC and Coot.
8)Validation of the final results.
Kind regards,
Marin van Heel
--
================================================================
Prof Dr Ir Marin van Heel
Professor of Cryo-EM Data Processing
Leiden University
NeCEN Building Room 05.27
Einsteinweg 55
2333 CC Leiden
The Netherlands
Tel. NL: +31(0)715271424 // Mobile NL: +31(0)652736618
Skype: Marin.van.Heel
email: marin.vanheel(A_T)gmail.com
and: mvh.office(A_T)gmail.com
----------------------------------------------
Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
Biochemistry Building (Room 512)
South Kensington Campus
London SW7 2AZ, UK
email: m.vanheel(A_T)ic.ac.uk
Tel. UK: +44(0)2075945316 //Mobile: +44(0)7941540625
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Visiting Professor at:
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/ABTLuS, Campinas, Brazil
Brazilian mobile phone +55-19-983189143
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