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<div class="moz-forward-container">NOTE: We have experienced a very
strong interest in the 7th Brazil school since its first
announcement. The school is already likely to be oversubscribed so
don't delay your registration!<br>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:107%;font-family:
"Times New Roman",serif"><span
style="mso-spacerun:yes"> </span><o:p></o:p></span></p>
<p class="MsoNormal"><u><span
style="font-size:12.0pt;line-height:107%;
font-family:"Times New Roman",serif">Second
announcement:<o:p></o:p></span></u></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:107%;font-family:
"Times New Roman",serif"><br>
<u>THE SEVENTH BRAZIL SCHOOL FOR SINGLE PARTICLE CRYO-EM.</u><o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:107%;font-family:
"Times New Roman",serif">11-23 August 2016 (São
Paulo, Brazil) (<a moz-do-not-send="true"
class="moz-txt-link-abbreviated"
href="http://www.single-particles.org/school">www.single-particles.org/school</a>).<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:107%;font-family:
"Times New Roman",serif">Our single-particle school
is a unique intensive 12-day hands-on course/workshop, in
which theory and practice are tightly intertwined. The
understanding of the principles of cryo-EM data processing
stands central in this event and a few pioneers will explain
the approaches to the participants to the level where they can
apply it themselves. The spectrum of topics covered suits
attendees who are new to the field as well as those for whom
the cryo-EM technique is of growing importance and who wish to
acquire an in-depth understanding of its fundamental
principles.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:107%;font-family:
"Times New Roman",serif">The final days of the
meeting will be devoted to advanced topics such as
high-resolution refinements and the processing of
heterogeneous data sets ("4D cryo–EM”). Advanced topics will
include:<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpFirst"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">1)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">Optimizing data collection with direct
electrons detectors and exploiting movies. <o:p></o:p></span></p>
<p class="MsoListParagraphCxSpMiddle"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">2)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">Full data set automatic CTF correction.<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpMiddle"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">3)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">Correction of anisotropic
magnification.<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpMiddle"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">4)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">Reaching atomic resolution without
reference bias: alignment-by-classification (ABC) refinement
procedures.<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpMiddle"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">5)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">Unbiased "Random Startup" of 3D and 4D
reconstructions.<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpMiddle"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">6)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">4D refinement procedures for high
resolution results using IMAGIC-4D and/or Frealign.<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpMiddle"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">7)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">Fitting and refining atomic coordinates
in high-resolution cryo-EM maps (~4Å or better) using programs
like REFMAC and Coot.<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpMiddle"
style="text-indent:-18.0pt;mso-list:l0 level1 lfo1"><!--[if !supportLists]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif; mso-fareast-font-family:"Times New
Roman""><span style="mso-list:Ignore">8)<span
style="font:7.0pt "Times New Roman""> </span></span></span><!--[endif]--><span
style="font-size:12.0pt;line-height:107%;font-family:"Times
New Roman",serif">Validation of the final results.<o:p></o:p></span></p>
<p class="MsoListParagraphCxSpLast"><span
style="font-size:12.0pt;line-height:
107%;font-family:"Times New Roman",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:107%;font-family:
"Times New Roman",serif">Kind regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:107%;font-family:
"Times New Roman",serif">Marin van Heel<o:p></o:p></span></p>
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<pre class="moz-signature" cols="72">--
================================================================
Prof Dr Ir Marin van Heel
Professor of Cryo-EM Data Processing
Leiden University
NeCEN Building Room 05.27
Einsteinweg 55
2333 CC Leiden
The Netherlands
Tel. NL: +31(0)715271424 // Mobile NL: +31(0)652736618
Skype: Marin.van.Heel
email: marin.vanheel(A_T)gmail.com
and: mvh.office(A_T)gmail.com
----------------------------------------------
Emeritus Professor of Structural Biology
Imperial College London
Faculty of Natural Sciences
Biochemistry Building (Room 512)
South Kensington Campus
London SW7 2AZ, UK
email: m.vanheel(A_T)ic.ac.uk
Tel. UK: +44(0)2075945316 //Mobile: +44(0)7941540625
----------------------------------------------
Visiting Professor at:
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/ABTLuS, Campinas, Brazil
Brazilian mobile phone +55-19-983189143
------------------------------------------------------------------
I receive many emails per day and, although I try,
there is no guarantee that I will actually read each incoming email.
Moreover, our Spam filters can be strikt and sometimes make
legitimate emails disappear (try the gmail accounts, alternatively)</pre>
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