[3dem] plunge with vitrobot

[Peter Shen]

Hi Xiaofeng,
I am familiar with this type of ice. It occurs when the droplet is poorly adsorbed across the grid surface. As Bob mentioned, depending on your particle, this type of suspension can be a great thing. I have found that applying the sample to both sides of the grid helps make this type of ice more uniform throughout the surface.
My two cents,Peter

Date: Sun, 25 May 2014 17:29:13 -0400
From: xiaofeng at brandeis.edu
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] plunge with vitrobot


Dear all,

Recently we had plunge problem by using vitrobot. Two images are attached: there is always a big droplet in the middle of square, and the ice is very thick at its neighboring area. We varied the GD from 10s to 40s at 24mA, but the grids look similar. The buffer doesn't contain any salt or sugar.


Look forward to hearing any suggestions or experiences.

Thanks,
Xiaofeng



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